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NCOA7 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - NCOA7 Antibody (N-term) AP9535a
    All lanes : Anti-NCOA7 Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 106 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - NCOA7 Antibody (N-term) AP9535a
    NCOA7 Antibody (N-term) (Cat. #AP9535a) western blot analysis in Hela,MDA-MB-453 cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the NCOA7 antibody detected the NCOA7 protein (arrow).
  • 14 - NCOA7 Antibody (N-term) AP9535a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with NCOA7 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - NCOA7 Antibody (N-term) AP9535a
    NCOA7 Antibody (N-term) (Cat. #AP9535a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB, E
Primary Accession Q8NI08
Other Accession Q6DFV7
Reactivity Human, Mouse
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 106162 Da
Additional info
Gene ID 135112
Other Names Nuclear receptor coactivator 7, 140 kDa estrogen receptor-associated protein, Estrogen nuclear receptor coactivator 1, NCOA7, ERAP140, ESNA1
Target/Specificity This NCOA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-103 amino acids from the N-terminal region of human NCOA7.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNCOA7 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCOA7
Synonyms ERAP140, ESNA1
Function Enhances the transcriptional activities of several nuclear receptors. Involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA.
Cellular Location Nucleus.
Tissue Location Highly expressed in brain. Weakly expressed in mammary gland, ovary, uterus, prostate, stomach, bladder, spinal cord and pancreas. Expressed in cancer cell line
Research Areas

BACKGROUND

NCOA7 enhances the transcriptional activities of several nuclear receptors. It is involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA.

REFERENCES

?Arai, H., et al. Oncol. Rep. 19(6):1381-1388(2008)
?Liu, Y., et al. Schizophr. Res. 89 (1-3), 360-361 (2007) :
?Durand, M., et al. BMC Cell Biol. 8, 13 (2007) :
?Mungall, A.J., et al. Nature 425(6960):805-811(2003)
?Ohira, M., et al. Cancer Lett. 197 (1-2), 63-68 (2003) :
?Rushing, S.R., et al. Arch. Biochem. Biophys. 403(2):189-201(2002)

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