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DIO3 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - DIO3 Antibody (C-term) AP9529b
    Anti-DIO3 Antibody (C-term) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - DIO3 Antibody (C-term) AP9529b
    Anti-DIO3 Antibody (C-term) at 1:2000 dilution + Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - DIO3 Antibody (C-term) AP9529b
    Anti-DIO3 Antibody (C-term) at 1:2000 dilution + Human placenta lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - DIO3 Antibody (C-term) AP9529b
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DIO3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P55073
Other Accession NP_001353
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 1735
Other Names Type III iodothyronine deiodinase, 5DIII, DIOIII, Type 3 DI, Type-III 5'-deiodinase, DIO3, ITDI3, TXDI3
Target/Specificity This DIO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the C-terminal region of human DIO3.
Dilution WB~~1:2000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDIO3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DIO3
Synonyms ITDI3, TXDI3
Function Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.
Cellular Location Cell membrane; Single-pass type II membrane protein. Endosome membrane; Single-pass type II membrane protein
Tissue Location Expressed in placenta and several fetal tissues
Research Areas

BACKGROUND

DIO3 belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.

REFERENCES

Bessho, K., et al. Eur. J. Pediatr. 169(2):215-221(2010)
Wallace, C., et al. Nat. Genet. 42(1):68-71(2010)
Aerts, G., et al. Endocrinology 150(11):5171-5180(2009)
Panicker, V., et al. J. Clin. Endocrinol. Metab. 94(5):1623-1629(2009)
Panicker, V., et al. J. Clin. Endocrinol. Metab. 93(8):3075-3081(2008)

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