- 文献引用 : 1
|Application ||WB, IHC-P, FC, E|
|Other Accession||P62323, P62320|
|Calculated MW||13916 Da|
|Other Names||Small nuclear ribonucleoprotein Sm D3, Sm-D3, snRNP core protein D3, SNRPD3|
|Target/Specificity||This SNRPD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-126 amino acids from the C-terminal region of human SNRPD3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SNRPD3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays role in pre-mRNA splicing as core component of the SMN-Sm complex that mediates spliceosomal snRNP assembly and as component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed:11991638, PubMed:18984161, PubMed:19325628, PubMed:25555158, PubMed:26912367, PubMed:28502770, PubMed:28781166, PubMed:28076346). Component of both the pre- catalytic spliceosome B complex and activated spliceosome C complexes (PubMed:11991638, PubMed:28502770, PubMed:28781166, PubMed:28076346). Is also a component of the minor U12 spliceosome (PubMed:15146077). As part of the U7 snRNP it is involved in histone pre-mRNA 3'-end processing (By similarity).|
|Cellular Location||Cytoplasm, cytosol. Nucleus Note=SMN-mediated assembly into core snRNPs occurs in the cytosol before SMN-mediated transport to the nucleus to be included in spliceosomes.|
Author : Weng MT1, Lee JH, Wei SC, Li Q, Shahamatdar S, Hsu D, Schetter AJ, Swatkoski S, Mannan P, Garfield S, Gucek M, Kim MK, Annunziata CM, Creighton CJ, Emanuele MJ, Harris CC, Sheu JC, Giaccone G, Luo J.
Proc Natl Acad Sci U S A. 2012 Dec 26;109(52):E3659-67. doi: 10.1073/pnas.1207673110. Epub 2012 Dec 10.
Provided below are standard protocols that you may find useful for product applications.
The protein belongs to the small nuclear ribonucleoprotein core protein family. It is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis.
Gonsalvez,G.B., et.al., J. Cell Biol. 178 (5), 733-740 (2007)
Ma,Y., et.al., Structure 13 (6), 883-892 (2005)
Mahler,M., et.al., Arthritis Res. Ther. 7 (1), R19-R29 (2005)