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>   首页   >   产品   >   一抗   >   精选抗体   >   G蛋白偶联受体抗体(GPCR)   >   PTGER3 Antibody (N-term)   

PTGER3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - PTGER3 Antibody (N-term) AP9132A
    All lanes : Anti-PTGER3 Antibody (N-term) at 1:1000 dilution Lane 1: Ramos whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: 293 whole cell lysate Lane 4: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - PTGER3 Antibody (N-term) AP9132A
    Anti-PTGER3 Antibody (N-term) at 1:500 dilution + Human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - PTGER3 Antibody (N-term) AP9132A
    Western blot analysis of PTGER3 Antibody (N-term) (Cat. #AP9132a) in Ramos cell line lysates (35ug/lane). PTGER3 (arrow) was detected using the purified Pab.
  • 4 - PTGER3 Antibody (N-term) AP9132A
    PTGER3 Antibody (N-term) (Cat. #AP9132a) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession P43115
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 43310 Da
Additional info
Gene ID 5733
Other Names Prostaglandin E2 receptor EP3 subtype, PGE receptor EP3 subtype, PGE2 receptor EP3 subtype, PGE2-R, Prostanoid EP3 receptor, PTGER3
Target/Specificity This PTGER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human PTGER3.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPTGER3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTGER3
Function Receptor for prostaglandin E2 (PGE2); the EP3 receptor may be involved in inhibition of gastric acid secretion, modulation of neurotransmitter release in central and peripheral neurons, inhibition of sodium and water reabsorption in kidney tubulus and contraction in uterine smooth muscle. The activity of this receptor can couple to both the inhibition of adenylate cyclase mediated by G-I proteins, and to an elevation of intracellular calcium. The various isoforms have identical ligand binding properties but can interact with different second messenger systems (By similarity).
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Expressed in small intestine, heart, pancreas, gastric fundic mucosa, mammary artery and pulmonary vessels
Research Areas

BACKGROUND

PTGER3 is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor may have many biological functions, which involve digestion, nervous system, kidney reabsorption, and uterine contraction activities. Studies of the mouse counterpart suggest that this receptor may also mediate adrenocorticotropic hormone response as well as fever generation in response to exogenous and endogenous stimuli.

REFERENCES

Schmid,A., et.al., Eur. J. Biochem. 228 (1), 23-30 (1995)
An,S., et.al., Biochemistry 33 (48), 14496-14502 (1994)

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