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CYP27B1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - CYP27B1 Antibody (C-term) AP9056B
    Overlay histogram showing Hela cells stained with AP9056b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP9056b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - CYP27B1 Antibody (C-term) AP9056B
    All lanes : Anti-CYP27B1 Antibody (C-term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: T47D whole cell lysate Lane 3: Rat kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - CYP27B1 Antibody (C-term) AP9056B
    All lanes : Anti-CYP27B1 Antibody (C-term) at 1:2000 dilution Lane 1: Human kidney lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - CYP27B1 Antibody (C-term) AP9056B
    Western blot analysis of lysate from T47D cell line, using CYP27B1 Antibody (C-term)(Cat. #AP9056b). AP9056b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • 14 - CYP27B1 Antibody (C-term) AP9056B
    CYP27B1 Antibody (C-term) (Cat. #AP9056B)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP27B1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, IHC-P, E
Primary Accession O15528
Reactivity Human, Rat
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 1594
Other Names 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial, 25-OHD-1 alpha-hydroxylase, 25-hydroxyvitamin D(3) 1-alpha-hydroxylase, VD3 1A hydroxylase, Calcidiol 1-monooxygenase, Cytochrome P450 subfamily XXVIIB polypeptide 1, Cytochrome P450C1 alpha, Cytochrome P450VD1-alpha, Cytochrome p450 27B1, CYP27B1, CYP1ALPHA, CYP27B
Target/Specificity This CYP27B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-508 amino acids from the C-terminal region of human CYP27B1.
Dilution FC~~1:25
WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYP27B1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYP27B1
Synonyms CYP1ALPHA, CYP27B
Function Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.
Cellular Location Mitochondrion membrane.
Tissue Location Kidney.
Research Areas
25(OH)D Is Effective to Repress Human Cholangiocarcinoma Cell Growth through the Conversion of 25(OH)D to 1α,25(OH)₂D₃.
Author : Chiang KC1, Yeh CN2, Huang CC3, Yeh TS4, S Pang JH5, Hsu JT6, Chen LW7, Kuo SF8, Kittaka A9, Chen TC10, Juang HH11.
Int J Mol Sci. 2016 Aug 12;17(8). pii: E1326. doi: 10.3390/ijms17081326.
27529229
Reduced sinonasal levels of 1α-hydroxylase are associated with worse quality of life in chronic rhinosinusitis with nasal polyps.
Author : Schlosser RJ1,2, Carroll WW1, Soler ZM1, Pasquini WN1, Mulligan JK1,2,3.
Int Forum Allergy Rhinol. 2015 Nov 17. doi: 10.1002/alr.21576. [Epub ahead of print]
26575398
Antenatal endotoxin disrupts lung vitamin D receptor and 25-hydroxyvitamin D 1α-hydroxylase expression in the developing rat.
Author : Mandell E1, Seedorf GJ2, Ryan S3, Gien J3, Cramer SD4, Abman SH2.
Am J Physiol Lung Cell Mol Physiol. 2015 Nov 1;309(9):L1018-26. doi: 10.1152/ajplung.00253.2015. Epub 2015 Sep 4.
26342089

BACKGROUND

CYP27B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

REFERENCES

Simon,K.C., et.al., Mult. Scler. 16 (2), 133-138 (2010)
Zhou,S., et.al., Endocrinology 151 (1), 14-22 (2010)

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