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ETV6 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ETV6 Antibody (N-term) AP8917a
    All lanes : Anti-ETV6 Antibody (N-term) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - ETV6 Antibody (N-term) AP8917a
    ETV6 Antibody (N-term) (Cat. #AP8917a) western blot analysis in U266 cell line lysates (35ug/lane).This demonstrates the ETV6 antibody detected the ETV6 protein (arrow).
  • 14 - ETV6 Antibody (N-term) AP8917a
    Formalin-fixed and paraffin-embedded human testis tissue reacted with ETV6 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - ETV6 Antibody (N-term) AP8917a
    ETV6 Antibody (N-term) (Cat. #AP8917a) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession P41212
Other Accession P97360, Q0VC65
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 53000 Da
Additional info
Gene ID 2120
Other Names Transcription factor ETV6, ETS translocation variant 6, ETS-related protein Tel1, Tel, ETV6, TEL, TEL1
Target/Specificity This ETV6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-111 amino acids from the N-terminal region of human ETV6.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsETV6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ETV6
Synonyms TEL, TEL1
Function Transcriptional repressor; binds to the DNA sequence 5'- CCGGAAGT-3'. Plays a role in hematopoiesis and malignant transformation.
Cellular Location Nucleus.
Tissue Location Ubiquitous.
Research Areas

BACKGROUND

ETV6 is an ETS family transcription factor. This protein contains two functional domains: a N-terminal pointed (PNT) domain that is involved in protein-protein interactions with itself and other proteins, and a C-terminal DNA-binding domain.

REFERENCES

Buijs,A., et.al., Oncogene 10 (8), 1511-1519 (1995)
Golub,T.R.,et.al., Proc. Natl. Acad. Sci. U.S.A. 92 (11), 4917-4921 (1995)

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