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ALG14 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ALG14 Antibody (Center) AP8903c
    Western blot analysis of ALG14 Antibody (Center) (Cat. #AP8903c) in NCI-H460 cell line lysates (35ug/lane). ALG14 (arrow) was detected using the purified Pab.
  • 14 - ALG14 Antibody (Center) AP8903c
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with ALG14 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - ALG14 Antibody (Center) AP8903c
    ALG14 Antibody (Center) (Cat. #AP8903c) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, IHC-P, E
Primary Accession Q96F25
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 199857
Other Names UDP-N-acetylglucosamine transferase subunit ALG14 homolog, ALG14
Target/Specificity This ALG14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-93 amino acids from the Central region of human ALG14.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALG14 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALG14
Function May be involved in protein N-glycosylation. May play a role in the second step of the dolichol-linked oligosaccharide pathway. May anchor the catalytic subunit ALG13 to the ER.
Cellular Location Endoplasmic reticulum membrane; Single-pass membrane protein
Research Areas
Congenital myasthenic syndromes due to mutations in ALG2 and ALG14.
Author : Cossins J1, Belaya K, Hicks D, Salih MA, Finlayson S, Carboni N, Liu WW, Maxwell S, Zoltowska K, Farsani GT, Laval S, Seidhamed MZ; WGS500 Consortium, Donnelly P, Bentley D, McGowan SJ, Müller J, Palace J, Lochmüller H, Beeson D.Collaborators
Brain. 2013 Mar;136(Pt 3):944-56. doi: 10.1093/brain/awt010. Epub 2013 Feb 11.
23404334

BACKGROUND

ALG14 is involved in protein N-glycosylation. It is essential for the second step of the dolichol-linked oligosaccharide pathway. It anchors the catalytic subunit ALG13 to the ER.

REFERENCES

Gao X.-D., et.al., J. Biol. Chem. 280:36254-36262(2005).

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