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IGH Antibody (C-Term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - IGH Antibody (C-Term) AP8783b
    Anti-IGH Antibody (C-Term) at 1:1000 dilution + A2058 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - IGH Antibody (C-Term) AP8783b
    Western blot analysis of IGH Antibody (C-Term) (Cat. #AP8783b) in HL-60, A2058 cell line lysates (35ug/lane). IGH (arrow) was detected using the purified Pab.
  • 14 - IGH Antibody (C-Term) AP8783b
    Formalin-fixed and paraffin-embedded human skin reacted with IGH Antibody (C-Term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - IGH Antibody (C-Term) AP8783b
    IGH Antibody (C-Term) (Cat. #AP8783b) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession P01876
Other Accession P01877
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 37655 Da
Additional info
Other Names Ig alpha-1 chain C region, IGHA1
Target/Specificity This IGH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 413-439 amino acids from the C-terminal region of human IGH.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIGH Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IGHA1
Function Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.
Research Areas

REFERENCES

Strausberg,R.L.,et.al., Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)

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