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DIO2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - DIO2 Antibody (Center) AP8718c
    All lanes : Anti-DIO2 Antibody (Center) at 1:500-2000 dilution Lane 1: Mouse heart tissue lysate Lane 2: Human skeletal muscle tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - DIO2 Antibody (Center) AP8718c
    Western blot analysis of lysates from human heart and human skeletal muscle tissue lysate (from left to right), using DIO2 Antibody (Center)(Cat. #AP8718c). AP8718c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • 1 - DIO2 Antibody (Center) AP8718c
    Western blot analysis of DIO2 Antibody (Center) (Cat. #AP8718c) in MCF-7 cell line lysates (35ug/lane). DIO2 (arrow) was detected using the purified Pab.
  • 14 - DIO2 Antibody (Center) AP8718c
    DIO2 Antibody (Center) (Cat. #AP8718c) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the mouse DIO2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - DIO2 Antibody (Center) AP8718c
    DIO2 Antibody (Center) (Cat. #AP8718c) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q92813
Other Accession Q6QN12
Reactivity Human, Mouse
Predicted Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 30552 Da
Additional info
Gene ID 1734
Other Names Type II iodothyronine deiodinase, 5DII, DIOII, Type 2 DI, Type-II 5'-deiodinase, DIO2, ITDI2, TXDI2
Target/Specificity This DIO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-191 amino acids from the Central region of human DIO2.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDIO2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DIO2
Synonyms ITDI2, TXDI2
Function Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.
Cellular Location Membrane; Single-pass membrane protein
Tissue Location Isoform 1 is expressed in the lung, trachea, kidney, heart, skeletal muscle, placenta, fetal brain and several regions of the adult brain (PubMed:8755651, PubMed:11165050) Isoform 2 is expressed in the brain, heart, kidney and trachea (PubMed:11165050).
Research Areas

BACKGROUND

DIO2 belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3).

REFERENCES

He,B.,et.al., Prog. Neuropsychopharmacol. Biol. Psychiatry 33 (6), 986-990 (2009)
Heemstra,K.A., et.al., J. Clin. Endocrinol. Metab. 94 (6), 2144-2150 (2009)

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