KLRC2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-KLRC2 Antibody (N-term) at 1:2000 dilution Lane 1: human uterus lysate Lane 2: CCRF-CEM whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded H.colon tissue reacted with KLRC2 Antibody (N-term) (Cat#AP8630a).
Western blot analysis of KLRC2 Antibody (N-term) (Cat. #AP8630a) in HL-60 cell line lysates (35ug/lane). KLRC2 (arrow) was detected using the purified Pab.
KLRC2 Antibody (N-term) (Cat. #AP8630a) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
KLRC2 Antibody (N-term) (Cat. #AP8630a) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsillitis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the KLRC2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IHC-P, E
Primary Accession	P26717
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit Ig

Additional info
Gene ID	3822
Other Names	NKG2-C type II integral membrane protein, CD159 antigen-like family member C, NK cell receptor C, NKG2-C-activating NK receptor, CD159c, KLRC2, NKG2C
Target/Specificity	This KLRC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human KLRC2.
Dilution	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	KLRC2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	KLRC2
Synonyms	NKG2C {ECO:0000303\|PubMed:18083576}
Function	Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells.
Cellular Location	Membrane; Single-pass type II membrane protein
Tissue Location	Natural killer cells.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Flow Cytomety Protocol

Cell Culture Protocol

KLRC2 plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells.

Seo,J., et.al., Tissue Antigens 70 (4), 307-313 (2007)
Park,K.S., et.al., Tissue Antigens 72 (4), 342-346 (2008)

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Cat# AP8630a

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