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HLA-B Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 59 - HLA-B Antibody (N-term) AP8616a
    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using AP8616a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - HLA-B Antibody (N-term) AP8616a
    All lanes : Anti-HLA-B Antibody (N-term) at 1:1000-2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Ramos whole cell lysate Lane 5: A375 whole cell lysate Lane 6: A2058 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 4 - HLA-B Antibody (N-term) AP8616a
    Flow cytometric analysis of human peripheral blood lymphocytes cells using HLA-B Antibody (N-term) (green, Cat#AP8616a) compared to an isotype control of rabbit IgG (blue). AP8616a was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, FC, WB, E
Primary Accession P03989
Other Accession P30490, P30488, P30487, P30486, P30485, P30483, P30481, P30479, Q04826, P18463, P30464, P30461
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Other Names HLA class I histocompatibility antigen, B-27 alpha chain, MHC class I antigen B*27, HLA-B, HLAB
Target/Specificity This HLA-B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human HLA-B.
Dilution IHC-P-Leica~~1:500
WB~~1:1000-2000
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHLA-B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HLA-B
Synonyms HLAB
Function Involved in the presentation of foreign antigens to the immune system.
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas

BACKGROUND

HLA-B is involved in the presentation of foreign antigens to the immune system.

REFERENCES

Higgins,C.M., et.al., Ann. Rheum. Dis. 51 (7), 855-862 (1992)
Guild,B.C.et.al., J. Biol. Chem. 259 (14), 9235-9240 (1984)
Orr,H.T., et.alt., Biochemistry 18 (25), 5711-5720 (1979)

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