|Application ||WB, IHC-P, FC, E|
|Other Accession||Q8R5K6, Q61471|
|Calculated MW||38155 Da|
|Other Names||Protein Tob1, Transducer of erbB-2 1, TOB1, TOB, TROB1|
|Target/Specificity||This TOB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human TOB1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TOB1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex (PubMed:8632892, PubMed:23236473). Mediates CPEB3-accelerated mRNA deadenylation by binding to CPEB3 and recruiting CNOT7 which leads to target mRNA deadenylation and decay (PubMed:21336257).|
|Cellular Location||Cytoplasm. Nucleus. Note=Only a small fraction localizes to the cytoplasm except in late S-phase where more than half of proteins become cytoplasmic.|
Provided below are standard protocols that you may find useful for product applications.
TOB1 is a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells.
Suzuki,T., et.al., Genes Dev. 16 (11), 1356-1370 (2002)
Yoshida,Y., et.al., Cell 103 (7), 1085-1097 (2000)