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ADH5 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ADH5 Antibody (Center) AP8562C
    All lanes : Anti-ADH5 Antibody (Center) at 1:4000 dilution Lane 1: K562 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - ADH5 Antibody (Center) AP8562C
    Formalin-fixed and paraffin-embedded H.placenta tissue reacted with ADH5 Antibody (Center) (Cat#AP8562c).
  • 14 - ADH5 Antibody (Center) AP8562C
    Formalin-fixed and paraffin-embedded H.breast tissue reacted with ADH5 Antibody (Center) (Cat#AP8562c).
  • 1 - ADH5 Antibody (Center) AP8562C
    Western blot analysis of ADH5 (arrow) using rabbit polyclonal ADH5 Antibody (Center) (Cat. #AP8562c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH5 gene.
  • 14 - ADH5 Antibody (Center) AP8562C
    Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADH5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - ADH5 Antibody (Center) AP8562C
    Flow cytometric analysis of K562 cells using ADH5 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, IHC-P, E
Primary Accession P11766
Other Accession O19053
Reactivity Human
Predicted Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 128
Other Names Alcohol dehydrogenase class-3, Alcohol dehydrogenase 5, Alcohol dehydrogenase class chi chain, Alcohol dehydrogenase class-III, Glutathione-dependent formaldehyde dehydrogenase, FALDH, FDH, GSH-FDH, 111-, S-(hydroxymethyl)glutathione dehydrogenase, ADH5 (HGNC:253), ADHX, FDH
Target/Specificity This ADH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-239 amino acids from the Central region of human ADH5.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADH5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADH5 (HGNC:253)
Synonyms ADHX, FDH
Function Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.
Cellular Location Cytoplasm.
Research Areas

BACKGROUND

ADH5 is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis.

REFERENCES

Martins-de-Souza,D., et.al., Eur Arch Psychiatry Clin Neurosci 259 (3), 151-163 (2009)
Iborra,F.J., et.al., J. Histochem. Cytochem. 40 (12), 1865-1878 (1992)

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