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DUSP8 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - DUSP8 Antibody (C-term) AP8451b
    Western blot analysis of anti-DUSP8 Pab(Cat. #AP8451b) in Hela cell line lysate (35ug/lane). DUSP8(arrow) was detected using the purified Pab.
  • 14 - DUSP8 Antibody (C-term) AP8451b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q13202
Other Accession NP_004411
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 65827 Da
Additional info
Gene ID 1850
Other Names Dual specificity protein phosphatase 8, Dual specificity protein phosphatase hVH-5, DUSP8, C11orf81, VH5
Target/Specificity This DUSP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 456-487 amino acids from the C-terminal region of human DUSP8.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDUSP8 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DUSP8
Synonyms C11orf81, VH5
Function Has phosphatase activity with synthetic phosphatase substrates and negatively regulates mitogen-activated protein kinase activity, presumably by catalysing their dephosphorylation. Expected to display protein phosphatase activity toward phosphotyrosine, phosphoserine and phosphothreonine residues.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:O09112}. Nucleus {ECO:0000250|UniProtKB:O09112}
Tissue Location Abundant in brain, heart and skeletal muscle.
Research Areas

BACKGROUND

DUSP8 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP8 inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin.

REFERENCES

Berger, I.R., et al., Cancer Genet. Cytogenet. 159(2):155-159 (2005).
Hink, R.L., et al., Genomics 8(3):305-312 (2003).
Nesbit, M.A., et al., Genomics 42(2):284-294 (1997).
Martell, K.J., et al., J. Neurochem. 65(4):1823-1833 (1995).

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