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PFKFB4 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - PFKFB4 Antibody (Center) AP8154c
    All lanes : Anti-PFKFB4 Antibody (Center) at 1:1000 dilution Lane 1: Raji, whole cell lysate Lane 2: Molt-4 whole cell lysate Lane 3: NCI-H1299 whole cell lysate Lane 4: 293 whole cell lysate Lane 5: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - PFKFB4 Antibody (Center) AP8154c
    The anti-PFKFB4 Pab (Cat. #AP8154c) is used in Western blot to detect PFKFB4 in mouse brain tissue lysate.
  • 14 - PFKFB4 Antibody (Center) AP8154c
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKFB4 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 产品详情
  • 文献引用 : 5
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q16877
Other Accession Q4R8B6
Reactivity Human, Mouse
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54040 Da
Additional info
Gene ID 5210
Other Names 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 4, 6PF-2-K/Fru-2, 6-P2ase 4, PFK/FBPase 4, 6PF-2-K/Fru-2, 6-P2ase testis-type isozyme, 6-phosphofructo-2-kinase, Fructose-2, 6-bisphosphatase, PFKFB4
Target/Specificity This PFKFB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-296 amino acids from the Central region of human PFKFB4.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPFKFB4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PFKFB4
Function Synthesis and degradation of fructose 2,6-bisphosphate.
Research Areas
6-Phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 is essential for p53-null cancer cells.
Author : Ros S1,2,Flöter J3,Kaymak I3,Da Costa C4,Houddane A5,Dubuis S6,Griffiths B1,Mitter R7,Walz S8,Blake S4,Behrens A4,9,Brindle KM2,Zamboni N6,Rider MH5,Schulze A1,3.
Oncogene. 2017 Jan 16. doi: 10.1038/onc.2016.477. [Epub ahead of print]
28092678
Sertoli-secreted FGF-2 induces PFKFB4 isozyme expression in mouse spermatogenic cells by activation of the MEK/ERK/CREB pathway.
Author : Gómez M, Manzano A, Figueras A, Viñals F, Ventura F, Rosa JL, Bartrons R, Navarro-Sabaté �?
Am J Physiol Endocrinol Metab. 2012 Sep 15;303(6):E695-707. doi: 10.1152/ajpendo.00381.2011. Epub 2012 Jul 17.
22811469
Functional metabolic screen identifies 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 as an important regulator of prostate cancer cell survival.
Author : Ros S1, Santos CR, Moco S, Baenke F, Kelly G, Howell M, Zamboni N, Schulze A.
Cancer Discov. 2012 Apr;2(4):328-43. doi: 10.1158/2159-8290.CD-11-0234. Epub 2012 Mar 22.
22576210
Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases.
Author : Yalcin A, Clem BF, Simmons A, Lane A, Nelson K, Clem AL, Brock E, Siow D, Wattenberg B, Telang S, Chesney J.
J Biol Chem. 2009 Sep 4;284(36):24223-32. doi: 10.1074/jbc.M109.016816. Epub 2009 May 27.
19473963
Ras inhibition in glioblastoma down-regulates hypoxia-inducible factor-1alpha, causing glycolysis shutdown and cell death.
Author : Blum R, Jacob-Hirsch J, Amariglio N, Rechavi G, Kloog Y.
Cancer Res. 2005 Feb 1;65(3):999-1006.
15705901

BACKGROUND

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

REFERENCES

Sakai, A., et al., J. Biochem. 119(3):506-511 (1996). Manzano, A., et al., Gene 229 (1-2), 83-89 (1999).

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2015-11-16 18:03:30
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Species tested
homo sapiens
Application tested
WB
Organization tested
Hela and HCT116
Barcode encoding
0 000004 011378
Brief protocol
HCT116 cell line 第一道为shGFP, 第二三四道为不同的shPFKFB4 cell line