- 文献引用 : 1
|Application ||WB, IHC-P, FC, E|
|Other Accession||P63102, P63101, Q5ZKC9, P63103|
|Predicted||Bovine, Chicken, Rat|
|Calculated MW||27745 Da|
|Other Names||14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ|
|Target/Specificity||This YWHAZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-93 amino acids from the Central region of human YWHAZ.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||YWHAZ Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.|
|Cellular Location||Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes|
Author : Joshi NR1, Su RW1, Chandramouli GV2, Khoo SK3, Jeong JW1, Young SL4, Lessey BA5, Fazleabas AT6.
Hum Reprod. 2015 Sep 14. pii: dev229. [Epub ahead of print]
Provided below are standard protocols that you may find useful for product applications.
YWHAZ belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.
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Nellist, M., et al., J. Biol. Chem. 277(42):39417-39424 (2002).