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YWHAZ Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - YWHAZ Antibody (Center) AP8152c
    Western blot analysis of YWHAZ Antibody (Center) (Cat. #AP8152c) in MDA-MB231, A2058 cell line and mouse cerebellum, brain tissue lysates (35ug/lane). YWHAZ (arrow) was detected using the purified Pab.
  • 14 - YWHAZ Antibody (Center) AP8152c
    YWHAZ Antibody (Center) (Cat. #AP8152c) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the YWHAZ Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - YWHAZ Antibody (Center) AP8152c
    YWHAZ Antibody (Center) (Cat. #AP8152c) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession P63104
Other Accession P63102, P63101, Q5ZKC9, P63103
Reactivity Human, Mouse
Predicted Bovine, Chicken, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 27745 Da
Additional info
Gene ID 7534
Other Names 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ
Target/Specificity This YWHAZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-93 amino acids from the Central region of human YWHAZ.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsYWHAZ Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name YWHAZ
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Cellular Location Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes
Research Areas
Altered expression of microRNA-451 in eutopic endometrium of baboons (Papio anubis) with endometriosis.
Author : Joshi NR1, Su RW1, Chandramouli GV2, Khoo SK3, Jeong JW1, Young SL4, Lessey BA5, Fazleabas AT6.
Hum Reprod. 2015 Sep 14. pii: dev229. [Epub ahead of print]
26370665

BACKGROUND

YWHAZ belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.

REFERENCES

Powell, D.W., et al., Mol. Cell. Biol. 23(15):5376-5387 (2003).
Zhu, P., et al., Biochem. Biophys. Res. Commun. 301(4):991-999 (2003).
Li, Y., et al., J. Biol. Chem. 277(47):44593-44596 (2002).
Wang, H., et al., J. Clin. Endocrinol. Metab. 87(6):2629-2634 (2002).
Nellist, M., et al., J. Biol. Chem. 277(42):39417-39424 (2002).

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