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HK1 (Hexokinase) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - HK1 (Hexokinase) Antibody (N-term) AP8141a
    All lanes : Anti-HK1 (Hexokinase) Antibody (N-term) at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 110 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - HK1 (Hexokinase) Antibody (N-term) AP8141a
    All lanes : Anti-hHK1-L93 at 1:2000 dilution Lane 1: C2C12 whole cell lysate Lane 2: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 102 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - HK1 (Hexokinase) Antibody (N-term) AP8141a
    Western blot analysis of HK1 (arrow) using HK1 Antibody (N-term) (Cat.#AP8141a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HK1 gene (Lane 2) (Origene Technologies).
  • 14 - HK1 (Hexokinase) Antibody (N-term) AP8141a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • 1 - HK1 (Hexokinase) Antibody (N-term) AP8141a
    HK1 Antibody (L93) (Cat. #AP8141a) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the HK1 antibody detected the HK1 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P19367
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 102486 Da
Additional info
Gene ID 3098
Other Names Hexokinase-1, Brain form hexokinase, Hexokinase type I, HK I, HK1
Target/Specificity This HK1 (Hexokinase) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-108 amino acids from the N-terminal region of human HK1 (Hexokinase).
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHK1 (Hexokinase) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HK1
Cellular Location Mitochondrion outer membrane. Note=Its hydrophobic N-terminal sequence may be involved in membrane binding
Tissue Location Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific
Research Areas
Quantitative changes in the mitochondrial proteome from subjects with mild cognitive impairment, early stage, and late stage Alzheimer's disease.
Author : Lynn BC, Wang J, Markesbery WR, Lovell MA.
J Alzheimers Dis. 2010;19(1):325-39. doi: 10.3233/JAD-2010-1254.
20061648

BACKGROUND

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. The hexokinase gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of the hexokinase gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. HK1 encodes the ubiquitously expressed isoform. Its 5' end includes an exon which is unique to this transcript and which encodes a distinct N-terminus that contains the porin binding domain (PBD). The porin binding domain mediates association with the mitochondrial membrane.

REFERENCES

van Wijk, R., et al., Blood 101(1):345-347 (2003).
Murakami, K., et al., Acta Haematol. 108(4):204-209 (2002).
Murakami, K., et al., Mol. Genet. Metab. 67(2):118-130 (1999).
Aleshin, A.E., et al., Structure 6(1):39-50 (1998).
Ruzzo, A., et al., Blood 91(1):363-364 (1998).

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