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HK2 (Hexokinase II) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - HK2 (Hexokinase II) Antibody (N-term) AP8140a
    HK2 Antibody (E106) (Cat. #AP8140a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the HK2 antibody detected the HK2 protein (arrow).
  • 14 - HK2 (Hexokinase II) Antibody (N-term) AP8140a
    Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with HK2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P52789
Other Accession P27881, Q1W674, O08528
Reactivity Human
Predicted Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 102380 Da
Additional info
Gene ID 3099
Other Names Hexokinase-2, Hexokinase type II, HK II, Muscle form hexokinase, HK2
Target/Specificity This HK2 (Hexokinase II) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-121 amino acids from the N-terminal region of human HK2 (Hexokinase II).
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHK2 (Hexokinase II) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HK2
Cellular Location Mitochondrion outer membrane. Note=Its hydrophobic N-terminal sequence may be involved in membrane binding.
Tissue Location Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle
Research Areas
Analyses of resected human brain metastases of breast cancer reveal the association between up-regulation of hexokinase 2 and poor prognosis.
Author : Palmieri D, Fitzgerald D, Shreeve SM, Hua E, Bronder JL, Weil RJ, Davis S, Stark AM, Merino MJ, Kurek R, Mehdorn HM, Davis G, Steinberg SM, Meltzer PS, Aldape K, Steeg PS.
Mol Cancer Res. 2009 Sep;7(9):1438-45. doi: 10.1158/1541-7786.MCR-09-0234. Epub 2009 Sep 1.
19723875

BACKGROUND

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase activity is involved in the first step in several metabolic pathways. HK3 is bound to the outer mitochondrial membrane. Its hydrophobic N-terminal sequence may be involved in membrane bindng. It is the predominant hexokinase isozyme expressed in insuline-responsive tissues such as skeletal muscle. The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated wiht the N-terminus. Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.

REFERENCES

Lehto, M., et al., Diabetologia 38(12):1466-1474 (1995).
Vidal-Puig, A., et al., Diabetes 44(3):340-346 (1995).
Laakso, M., et al., Diabetes 44(3):330-334 (1995).
Echwald, S.M., et al., Diabetes 44(3):347-353 (1995).
Shinohara, Y., et al., Cancer Lett. 82(1):27-32 (1994).

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