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TLK2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - TLK2 Antibody (Center) AP8102c
    Western blot analysis of anti-TLK2 Antibody (Center) (Cat.#AP8102c) in mouse testis tissue lysates (35ug/lane).TLK2(arrow) was detected using the purified Pab.
  • 1 - TLK2 Antibody (Center) AP8102c
    TLK2 Antibody (K155) (Cat. #AP8102c) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the TLK2 antibody detected the TLK2 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q86UE8
Other Accession Q9UKI7
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 87661 Da
Additional info
Gene ID 11011
Other Names Serine/threonine-protein kinase tousled-like 2, HsHPK, PKU-alpha, Tousled-like kinase 2, TLK2
Target/Specificity This TLK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 141-171 amino acids from the Central region of human TLK2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTLK2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TLK2
Function Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly. Negative regulator of amino acid starvation-induced autophagy.
Cellular Location Nucleus. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Note=Colocalizes with the cytoplasmic intermediate filament system during the G1 phase of the cell cycle. Present in the perinuclear region at S phase and in the nucleus at late G2
Tissue Location Ubiquitous. Detected in placenta, fetal liver, kidney, pancreas, heart and skeletal muscle. Highly expressed in testis. Detected in spleen, thymus, colon, ovary, small intestine, prostate and peripheral blood leukocytes
Research Areas

BACKGROUND

TLK2, a member of the Ser/Thr protein kinase family, is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. The TLK2 enzyme is cell-cycle regulated, with maximal activity in S-phase. It is inactivated by phosphorylation at Ser-750, potentially by CHK1. TLK2 heterodimerizes with TLK1. This nuclear protein is widely expressed, with presence in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle tissues, and in several adult cell lines.

REFERENCES

Groth, A., et al., EMBO J. 22(7):1676-1687 (2003). Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Sillje, H.H., et al., EMBO J. 18(20):5691-5702 (1999). Yamakawa, A., et al., Gene 202 (1-2), 193-201 (1997).

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