- 文献引用 : 4
|Application ||WB, IF, IHC-P-Leica, E|
|Reactivity||Human, Mouse, Rat|
|Other Names||Phosphoenolpyruvate carboxykinase, cytosolic [GTP], PEPCK-C, PCK1, PEPCK1|
|Target/Specificity||This PCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 592-622 amino acids from the C-terminal region of human PCK1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PCK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle. At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate.|
|Tissue Location||Major sites of expression are liver, kidney and adipocytes|
Author : Leal-Esteban LC1,Rothé B1,Fortier S1,Isenschmid M1,Constam DB1.
PLoS Genet. 2018 Jul 11;14(7):e1007487. doi: 10.1371/journal.pgen.1007487. eCollection 2018 Jul.
Author : Gummadi L1, Taylor L, Curthoys NP.
Am J Physiol Renal Physiol. 2012 Dec 1;303(11):F1545-54. doi: 10.1152/ajprenal.00400.2012. Epub 2012 Sep 26.
Author : Berger K, Lindh R, Wierup N, Zmuda-Trzebiatowska E, Lindqvist A, Manganiello VC, Degerman E.
PLoS One. 2009;4(3):e4671. doi: 10.1371/journal.pone.0004671. Epub 2009 Mar 5.
Author : Franckhauser S1, Muñoz S, Elias I, Ferre T, Bosch F.
Diabetes. 2006 Feb;55(2):273-80.
Provided below are standard protocols that you may find useful for product applications.
This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.
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