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PCK1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PCK1 Antibody (C-term) AP8093b
    All lanes : Anti-PCK1 Antibody (C-term) at 1:2000 dilution Lane 1: Human liver lysate Lane 2: Human kidney lysate Lane 3: NCI-H460 whole cell lysate Lane 4: Mouse liver lysate Lane 5: Mouse kidney lysate Lane 6: Rat kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - PCK1 Antibody (C-term) AP8093b
    Western blot analysis of PCK1 Antibody (C-term) (Cat. #AP8093b) in rat primary hepatocyte cell line lysates. PCK1 (arrow) was detected using the purified Pab.
  • 3 - PCK1 Antibody (C-term) AP8093b
    Confocal immunofluorescent analysis of PCK1 Antibody (C-term)(Cat#AP8093b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
  • 1 - PCK1 Antibody (C-term) AP8093b
    The anti-PCK1 Pab (Cat. #AP8093b) is used in Western blot to detect PCK1 in mouse liver (Lane 1) and mouse small intestine tissue lysates (Lane 2).
  • 14 - PCK1 Antibody (C-term) AP8093b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P, E
Primary Accession P35558
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 5105
Other Names Phosphoenolpyruvate carboxykinase, cytosolic [GTP], PEPCK-C, PCK1, PEPCK1
Target/Specificity This PCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 592-622 amino acids from the C-terminal region of human PCK1.
Dilution WB~~1:1000
IF~~1:10~50
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCK1
Synonyms PEPCK1
Function Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Cellular Location Cytoplasm.
Tissue Location Major sites of expression are liver, kidney and adipocytes
Research Areas
Role of Bicaudal C1 in renal gluconeogenesis and its novel interaction with the CTLH complex.
Author : Leal-Esteban LC1,Rothé B1,Fortier S1,Isenschmid M1,Constam DB1.
PLoS Genet. 2018 Jul 11;14(7):e1007487. doi: 10.1371/journal.pgen.1007487. eCollection 2018 Jul.
29995892
Concurrent binding and modifications of AUF1 and HuR mediate the pH-responsive stabilization of phosphoenolpyruvate carboxykinase mRNA in kidney cells.
Author : Gummadi L1, Taylor L, Curthoys NP.
Am J Physiol Renal Physiol. 2012 Dec 1;303(11):F1545-54. doi: 10.1152/ajprenal.00400.2012. Epub 2012 Sep 26.
23019227
Phosphodiesterase 3B is localized in caveolae and smooth ER in mouse hepatocytes and is important in the regulation of glucose and lipid metabolism.
Author : Berger K, Lindh R, Wierup N, Zmuda-Trzebiatowska E, Lindqvist A, Manganiello VC, Degerman E.
PLoS One. 2009;4(3):e4671. doi: 10.1371/journal.pone.0004671. Epub 2009 Mar 5.
19262749
Adipose overexpression of phosphoenolpyruvate carboxykinase leads to high susceptibility to diet-induced insulin resistance and obesity.
Author : Franckhauser S1, Muñoz S, Elias I, Ferre T, Bosch F.
Diabetes. 2006 Feb;55(2):273-80.
16443757

BACKGROUND

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.

REFERENCES

Dunten, P., et al., J. Mol. Biol. 316(2):257-264 (2002).
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Deloukas, P., et al., Nature 414(6866):865-871 (2001).
O'Brien, R.M., et al., Biochim. Biophys. Acta 1264(3):284-288 (1995).
Ting, C.N., et al., Genomics 16(3):698-706 (1993).

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