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TRPM7 (CHAK1) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - TRPM7 (CHAK1) Antibody (C-term) AP8052b
    Western blot analysis of anti-CHAK1 Antibody (C-term) (Cat.#AP8052b) in mouse kidney tissue lysates (35ug/lane). CHAK1 (arrow) was detected using the purified Pab.
  • 14 - TRPM7 (CHAK1) Antibody (C-term) AP8052b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • 1 - TRPM7 (CHAK1) Antibody (C-term) AP8052b
    CHAK1 Antibody (F1835) (Cat. #AP8052b) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHAK1 antibody detected the CHAK1 protein (arrow).
  • 产品详情
  • 文献引用 : 3
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q96QT4
Other Accession Q9BXB2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 212697 Da
Additional info
Gene ID 54822
Other Names Transient receptor potential cation channel subfamily M member 7, Channel-kinase 1, Long transient receptor potential channel 7, LTrpC-7, LTrpC7, TRPM7, CHAK1, LTRPC7
Target/Specificity This TRPM7 (CHAK1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1821-1850 amino acids from the C-terminal region of human TRPM7 (CHAK1).
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTRPM7 (CHAK1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRPM7
Synonyms CHAK1, LTRPC7
Function Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF- induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
Cellular Location Membrane; Multi-pass membrane protein
Research Areas
Cyanidin Increases the Expression of Mg2+ Transport Carriers Mediated by the Activation of PPARα in Colonic Epithelial MCE301 Cells.
Author : Takashina Y1,Manabe A2,Tabuchi Y3,Ikari A4.
Nutrients. 2019 Mar 16;11(3). pii: E641. doi: 10.3390/nu11030641.
30884817
Zinc-induced neurotoxicity mediated by transient receptor potential melastatin 7 channels.
Author : Inoue K, Branigan D, Xiong ZG.
J Biol Chem. 2010 Mar 5;285(10):7430-9. doi: 10.1074/jbc.M109.040485. Epub 2010 Jan 4.
20048154
Expression of ion channels of the TRP family in articular chondrocytes from osteoarthritic patients: changes between native and in vitro propagated chondrocytes.
Author : Gavenis K, Schumacher C, Schneider U, Eisfeld J, Mollenhauer J, Schmidt-Rohlfing B.
Mol Cell Biochem. 2009 Jan;321(1-2):135-43. doi: 10.1007/s11010-008-9927-x. Epub 2008 Oct 4.
18836817

BACKGROUND

TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.[supplied by OMIM]

REFERENCES

Blume-Jensen P, et al. Nature 2001. 411: 355.
Cantrell D, J. Cell Sci. 2001. 114: 1439.
Jhiang S Oncogene 2000. 19: 5590.
Manning G, et al. Science 2002. 298: 1912.
Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359.
Robertson, S. et al. Trends Genet. 2000. 16: 368.
Robinson D, et al. Oncogene 2000. 19: 5548.
Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889.
Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561.
Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.

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