- 文献引用 : 3
|Application ||WB, IHC-P, E|
|Calculated MW||212697 Da|
|Other Names||Transient receptor potential cation channel subfamily M member 7, Channel-kinase 1, Long transient receptor potential channel 7, LTrpC-7, LTrpC7, TRPM7, CHAK1, LTRPC7|
|Target/Specificity||This TRPM7 (CHAK1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1821-1850 amino acids from the C-terminal region of human TRPM7 (CHAK1).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TRPM7 (CHAK1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF- induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).|
|Cellular Location||Membrane; Multi-pass membrane protein|
Author : Takashina Y1,Manabe A2,Tabuchi Y3,Ikari A4.
Nutrients. 2019 Mar 16;11(3). pii: E641. doi: 10.3390/nu11030641.
Author : Inoue K, Branigan D, Xiong ZG.
J Biol Chem. 2010 Mar 5;285(10):7430-9. doi: 10.1074/jbc.M109.040485. Epub 2010 Jan 4.
Author : Gavenis K, Schumacher C, Schneider U, Eisfeld J, Mollenhauer J, Schmidt-Rohlfing B.
Mol Cell Biochem. 2009 Jan;321(1-2):135-43. doi: 10.1007/s11010-008-9927-x. Epub 2008 Oct 4.
Provided below are standard protocols that you may find useful for product applications.
TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.[supplied by OMIM]
Blume-Jensen P, et al. Nature 2001. 411: 355.
Cantrell D, J. Cell Sci. 2001. 114: 1439.
Jhiang S Oncogene 2000. 19: 5590.
Manning G, et al. Science 2002. 298: 1912.
Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359.
Robertson, S. et al. Trends Genet. 2000. 16: 368.
Robinson D, et al. Oncogene 2000. 19: 5548.
Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889.
Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561.
Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.