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PI4KCB Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - PI4KCB Antibody (N-term) AP8030a
    Western blot analysis of anti-PI4KCB Pab (Cat. #AP8030a) in A375 cell lysate. PI4KCB (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • 14 - PI4KCB Antibody (N-term) AP8030a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9UBF8
Other Accession O08561, Q8BKC8, O02810, Q5VWB9
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 91379 Da
Additional info
Gene ID 5298
Other Names Phosphatidylinositol 4-kinase beta, PI4K-beta, PI4Kbeta, PtdIns 4-kinase beta, NPIK, PI4K92, PI4KB, PIK4CB
Target/Specificity This PI4KCB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-51 amino acids from the N-terminal region of human PI4KCB.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPI4KCB Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PI4KB (HGNC:8984)
Synonyms PIK4CB
Function Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol- 1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity).
Cellular Location Endomembrane system. Mitochondrion outer membrane; Peripheral membrane protein. Rough endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus. Golgi apparatus membrane. Cytoplasm, perinuclear region Note=Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) on the Golgi complex Recruited to the Golgi apparatus membrane by ACBD3, GGA2 is also involved in the recruitment.
Tissue Location Widely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver.
Research Areas
Phosphatidylinositol 4-kinase III beta is essential for replication of human rhinovirus and its inhibition causes a lethal phenotype in vivo.
Author : Spickler C1, Lippens J, Laberge MK, Desmeules S, Bellavance É, Garneau M, Guo T, Hucke O, Leyssen P, Neyts J, Vaillancourt FH, Décor A, O\'Meara J, Franti M, Gauthier A.
Antimicrob Agents Chemother. 2013 Jul;57(7):3358-68. doi: 10.1128/AAC.00303-13. Epub 2013 May 6.
23650168

BACKGROUND

Phosphoinositides are pivotal precursors to important second messengers and as signaling and molecules. Phosphatidylinositol 4-kinases (PI4Ks) are are crucial regulators of the phosphoinsitide cascade. PI4KCB is a wortmannin-sensitive PI 4-kinase responsible for regulating the synthesis of agonist-sensitive pools of polyphosphoinositides. The cellular reservoir of PI4KCB is predominantly cytosolic, however the protein is is activated strongly by recruitment to the membrane to stimulate phosphatidylinositol 4,5-bisphosphate synthesis at the plasma membrane. PI4KCB contains an N-terminal lipid kinase unique domain, which is shared by members of both the PI3 kinase and PI4 kinase families, and a C-terminal catalytic domain, which defines this protein as a member of a much larger protein/lipid kinase family.

REFERENCES

Wei, Y.J., et al., J. Biol. Chem. 277(48):46586-46593 (2002).
Balla, A., et al., J. Biol. Chem. 277(22):20041-20050 (2002).
Sorensen, S.D., et al., Mol. Pharmacol. 53(5):827-836 (1998).
Saito, T., et al., DNA Res. 4(4):301-305 (1997).
Suzuki, K., et al., DNA Res. 4(4):273-280 (1997).

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