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MARK2 (EMK) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - MARK2 (EMK) Antibody (C-term) AP8003a
    Western blot analysis of anti-EMK Antibody (C-term)(Cat.#AP8003a) in mouse thymus tissue lysates (35ug/lane). EMK(arrow) was detected using the purified Pab.
  • 14 - MARK2 (EMK) Antibody (C-term) AP8003a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • 1 - MARK2 (EMK) Antibody (C-term) AP8003a
    EMK Antibody (V581) (Cat. #AP8003a) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the EMK antibody detected the EMK protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q7KZI7
Other Accession O08679, Q05512, Q15524
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 87911 Da
Additional info
Gene ID 2011
Other Names Serine/threonine-protein kinase MARK2, ELKL motif kinase 1, EMK-1, MAP/microtubule affinity-regulating kinase 2, PAR1 homolog, PAR1 homolog b, Par-1b, Par1b, MARK2 {ECO:0000312|EMBL:AAH087712}, EMK1
Target/Specificity This MARK2 (EMK) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 600-630 amino acids from the C-terminal region of human MARK2 (EMK).
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMARK2 (EMK) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MARK2 {ECO:0000312|EMBL:AAH08771.2}
Synonyms EMK1
Function Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4, MAPT/TAU, and RAB11FIP2. Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule- dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.
Cellular Location Cell membrane; Peripheral membrane protein. Cytoplasm. Lateral cell membrane. Cytoplasm, cytoskeleton Note=Phosphorylation at Thr-596 by PRKCZ/aPKC and subsequent interaction with 14-3-3 protein YWHAZ promotes relocation from the cell membrane to the cytoplasm
Tissue Location High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
Research Areas
Adaptive responses to alloxan-induced mild oxidative stress ameliorate certain tauopathy phenotypes.
Author : Yoshiike Y, Yamashita S, Mizoroki T, Maeda S, Murayama M, Kimura T, Sahara N, Soeda Y, Takashima A.
Aging Cell. 2012 Feb;11(1):51-62. doi: 10.1111/j.1474-9726.2011.00756.x. Epub 2011 Nov 15.
21981382

BACKGROUND

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

REFERENCES

Blume-Jensen P, et al. Nature 2001. 411: 355.
Cantrell D, J. Cell Sci. 2001. 114: 1439.
Jhiang S Oncogene 2000. 19: 5590.
Manning G, et al. Science 2002. 298: 1912.
Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359.
Robertson, S. et al. Trends Genet. 2000. 16: 368.
Robinson D, et al. Oncogene 2000. 19: 5548.
Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889.
Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561.
Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.

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