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>   首页   >   产品   >   一抗   >   精选抗体   >   磷酸化抗体   >   Phospho-TNIK(S764) Antibody   

Phospho-TNIK(S764) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 6 - Phospho-TNIK(S764) Antibody AP7970d
    Dot blot analysis of Phospho-TNIK-pS764 Pab (Cat. #AP3276a) and TNIK-pS764 Pab (AP7970d)on nitrocellulose membrane. 50ng of Phospho-peptide (BP3276a) or Non Phospho-peptide (BP7970d) per dot were adsorbed. Antobodies working concentration was 0.5ug per ml. P-Pab: phosphorylated antibody; NP-Pab: non-phophorylated antibody; P-Pep: phospho-peptide; NP-Pep: non-phospho-peptide.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
DB, E
Primary Accession Q9UKE5
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 23043
Other Names TRAF2 and NCK-interacting protein kinase, TNIK, KIAA0551
Target/Specificity This TNIK Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S764 of human TNIK.
Dilution DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-TNIK(S764) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TNIK
Synonyms KIAA0551
Function Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. Phosphorylates SMAD1 on Thr-322.
Cellular Location Nucleus. Cytoplasm. Recycling endosome. Cytoplasm, cytoskeleton. Note=Associated with recycling endosomes and the cytoskeletal fraction upon RAP2A overexpression
Tissue Location Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues
Research Areas

BACKGROUND

Germinal center kinases (GCKs), such as TNIK, are characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function (Fu et al., 1999 [PubMed 10521462]).[supplied by OMIM]

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