- 文献引用 : 4
|Application ||WB, IHC-P, E|
|Other Accession||O88506, Q9Z1W9|
|Calculated MW||59474 Da|
|Other Names||STE20/SPS1-related proline-alanine-rich protein kinase, Ste-20-related kinase, DCHT, Serine/threonine-protein kinase 39, STK39, SPAK|
|Target/Specificity||This SPAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-376 amino acids from the Central region of human SPAK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPAK Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May act as a mediator of stress-activated signals. Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities by the WNK scaffolds, probably through phosphorylation. Phosphorylates RELT.|
|Cellular Location||Cytoplasm. Nucleus. Note=Nucleus when caspase-cleaved.|
|Tissue Location||Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis|
Author : Yang L, Cai X, Zhou J, Chen S, Chen Y, Chen Z, Wang Q, Fang Z, Zhou L.
PLoS One. 2013 Sep 13;8(9):e74614. doi: 10.1371/journal.pone.0074614.
Author : Wang Y, O'Connell JR, McArdle PF, Wade JB, Dorff SE, Shah SJ, Shi X, Pan L, Rampersaud E, Shen H, Kim JD, Subramanya AR, Steinle NI, Parsa A, Ober CC, Welling PA, Chakravarti A, Weder AB, Cooper RS, Mitchell BD, Shuldiner AR, Chang YP.
Proc Natl Acad Sci U S A. 2009 Jan 6;106(1):226-31. doi: 10.1073/pnas.0808358106. Epub 2008 Dec 29.
Author : Smith L, Smallwood N, Altman A, Liedtke CM.
J Biol Chem. 2008 Aug 8;283(32):22147-56. doi: 10.1074/jbc.M801752200. Epub 2008 Jun 11.
Author : Liedtke CM, Wang X, Smallwood ND.
J Biol Chem. 2005 Jul 8;280(27):25491-8. Epub 2005 May 16.
Provided below are standard protocols that you may find useful for product applications.
SPAK is a serine/threonine kinase containing an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain, a nuclear localization signal, a consensus caspase cleavage recognition motif, and a C-terminal region. Northern blot analysis detects ubiquitous expression, most abundantly in brain and pancreas. SPAK can phosphorylate itself and an exogenous substrate in vitro. SPAK immunoprecipitates from transfected mammalian cells in a complex with another serine/threonine kinase that phosphorylates catalytically inactive SPAK. SPAK activates the p38 MAP kinase pathway in cotransfection assays. Full-length SPAK is expressed in the cytoplasm in transfected cells, while a mutant corresponding to caspase-cleaved STK39 localizes predominantly in the nucleus.
Dowd, B.F., et al., J. Biol. Chem. 278(30):27347-27353 (2003).
Johnston, A.M., et al., Oncogene 19(37):4290-4297 (2000).