- 文献引用 : 3
|Application ||WB, IHC-P, E|
|Calculated MW||88393 Da|
|Other Names||Myosin light chain kinase 3, Cardiac-MyBP-C-associated Ca/CaM kinase, Cardiac-MLCK, MYLK3, MLCK|
|Target/Specificity||This MYLK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from the N-terminal region of human MYLK3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MYLK3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility (By similarity).|
|Tissue Location||Restricted to heart.|
Author : Gao MH1,Lai NC1,Giamouridis D1,Kim YC1,Tan Z1,Guo T1,Dillmann WH1,Suarez J1,Hammond HK1.
JACC Basic Transl Sci. 2016 Dec;1(7):617-629. doi: 10.1016/j.jacbts.2016.08.004.
Author : Rossi JL1, Todd T, Daniels Z, Bazan NG, Belayev L.
J Neurotrauma. 2015 Feb 10. [Epub ahead of print]
Author : Rossi JL, Ralay Ranaivo H, Patel F, Chrzaszcz M, Venkatesan C, Wainwright MS.
J Neurosci Res. 2011 Jun;89(6):852-61. doi: 10.1002/jnr.22600. Epub 2011 Feb 24.
Provided below are standard protocols that you may find useful for product applications.
MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.
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