|Application ||WB, E|
|Calculated MW||74869 Da|
|Other Names||Serine/threonine-protein kinase PAK 6, PAK-5, p21-activated kinase 6, PAK-6, PAK6, PAK5|
|Target/Specificity||This PAK6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-146 amino acids from human PAK6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PAK6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD.|
|Cellular Location||Cytoplasm. Nucleus. Note=Cotranslocates into nucleus with AR in response to androgen induction|
|Tissue Location||Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast.|
The PAK6 protein shares a high degree of sequence similarity with p21-activated kinase (PAK) family members. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. PAK6 was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. The p21-activated protein kinase 6 gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen.
Ching, Y.P., et al., J. Biol. Chem. 278(36):33621-33624 (2003). Pandey, A., et al., Oncogene 21(24):3939-3948 (2002). Yang, F., et al., J. Biol. Chem. 276(18):15345-15353 (2001).