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GAPDH Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - GAPDH Antibody (N-term) AP7873a
    All lanes : Anti-GAPDH Antibody (N-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - GAPDH Antibody (N-term) AP7873a
    Western blot analysis of lysate from A375 cell line, using GAPDH Antibody (N-term)(Cat. #AP7873a). AP7873a was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • 1 - GAPDH Antibody (N-term) AP7873a
    Western blot analysis of lysates from Hela,HUVEC cell line (from left to right),using GAPDH Antibody (N-term)(Cat. #AP7873a).AP7873a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
  • 14 - GAPDH Antibody (N-term) AP7873a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GAPDH antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 3 - GAPDH Antibody (N-term) AP7873a
    GAPDH Antibody (N-term) (Cat. # AP7873a) confocal immunofluorescent analysis with Hela cell. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. DAPI was used to stain the cell nuclear (blue).
  • 3 - GAPDH Antibody (N-term) AP7873a
    Confocal immunofluorescent analysis of GAPDH Antibody (N-term)(Cat#AP7873a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
  • 产品详情
  • 文献引用 : 17
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, WB, E
Primary Accession P04406
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 2597
Other Names Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, 2699-, GAPDH, GAPD
Target/Specificity This GAPDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the N-terminal region of human GAPDH.
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGAPDH Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GAPDH
Synonyms GAPD
Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Cellular Location Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton. Note=Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
Research Areas
Wnt pathway is involved in 5-FU drug resistance of colorectal cancer cells.
Author : He L1,Zhu H1,Zhou S1,Wu T1,Wu H1,Yang H1,Mao H1,SekharKathera C1,Janardhan A1,Edick AM2,Zhang A3,Hu Z1,Pan F1,Guo Z4.
Exp Mol Med. 2018 Aug 14;50(8):101. doi: 10.1038/s12276-018-0128-8.
30111797
Atorvastatin ameliorates early brain injury through inhibition of apoptosis and ER stress in a rat model of subarachnoid hemorrhage.
Author : Qi W1,Cao D1,Li Y1,Peng A1,Wang Y1,Gao K1,Tao C1,Wu Y2.
Biosci Rep. 2018 Mar 28. pii: BSR20171035. doi: 10.1042/BSR20171035. [Epub ahead of print]
29592873
PSMD7 downregulation induces apoptosis and suppresses tumorigenesis of esophageal squamous cell carcinoma via the mTOR/p70S6K pathway.
Author : Shi K1,Zhang JZ1,Zhao RL2,3,Yang L4,Guo D1.
FEBS Open Bio. 2018 Mar 7;8(4):533-543. doi: 10.1002/2211-5463.12394. eCollection 2018 Apr.
29632807
Migration ability and Toll-like receptor expression of human mesenchymal stem cells improves significantly after three-dimensional culture.
Author : Zhou P1,Liu Z1,Li X1,Zhang B1,Wang X1,Lan J1,Shi Q2,Li D3,Ju X4.
Biochem Biophys Res Commun. 2017 Jul 19. pii: S0006-291X(17)31453-5. doi: 10.1016/j.bbrc.2017.07.102. [Epub ahead of print]
28734835
Proteasome inhibitor MG132 induces thyroid cancer cell apoptosis by modulating the activity of transcription factor FOXO3a.
Author : Qiang W1,Sui F1,Ma J1,Li X1,Ren X1,Shao Y1,Liu J1,Guan H2,Shi B1,3,Hou P4,5.
Endocrine. 2017 Feb 20. doi: 10.1007/s12020-017-1256-y. [Epub ahead of print]
28220348
microRNA -140-5p inhibits colorectal cancer invasion and metastasis by targeting ADAMTS5 and IGFBP5.
Author : Yu L1,Lu Y1,2,Han X1,Zhao W1,Li J1,Mao J1,3,Wang B1,Shen J2,Fan S1,Wang L1,Wang M1,4,Li L1,3,Tang J1,4,Song B5,6.
Stem Cell Res Ther. 2016 Dec 1;7(1):180.
27906093
Increased expression of EHF via gene amplification contributes to the activation of HER family signaling and associates with poor survival in gastric cancer.
Author : Shi J1, Qu Y1, Li X1, Sui F1, Yao D2, Yang Q1, Shi B1,3, Ji M4, Hou P1,3.
Cell Death Dis. 2016 Oct 27;7(10):e2442. doi: 10.1038/cddis.2016.346.
27787520
Transient scrotal hyperthermia affects human sperm DNA integrity, sperm apoptosis, and sperm protein expression.
Author : Rao M1, Xia W1,2, Yang J1, Hu LX3, Hu SF1, Lei H1, Wu YQ1, Zhu CH1,2.
Andrology. 2016 Jul 13. doi: 10.1111/andr.12228. [Epub ahead of print]
27410176
The Ring Finger Protein RNF6 Induces Leukemia Cell Proliferation as a Direct Target of Pre-B-cell Leukemia Homeobox 1.
Author : Xu X1, Han K2, Tang X3, Zeng Y1, Lin X1, Zhao Y4, Zhang Z1, Cao B1, Wu D3, Mao X5.
J Biol Chem. 2016 Apr 29;291(18):9617-28. doi: 10.1074/jbc.M115.701979. Epub 2016 Mar 12.
26971355
miR-221/222 enhance the tumorigenicity of human breast cancer stem cells via modulation of PTEN/Akt pathway.
Author : Li B1, Lu Y2, Wang H3, Han X2, Mao J2, Li J2, Yu L2, Wang B2, Fan S2, Yu X2, Song B4.
Biomed Pharmacother. 2016 Apr;79:93-101. doi: 10.1016/j.biopha.2016.01.045. Epub 2016 Feb 17.
27044817
The effect of 3-bromopyruvate on human colorectal cancer cells is dependent on glucose concentration but not hexokinase II expression.
Author : Ho N1, Morrison J1, Silva A1, Coomber BL2.
Biosci Rep. 2016 Jan 6. pii: BSR20150267. [Epub ahead of print]
26740252
Prognostic significance of FAM3C in esophageal squamous cell carcinoma.
Author : Zhu YH1, Zhang B2, Li M3, Huang P4, Sun J5, Fu J6,7,8, Guan XY9,10.
Diagn Pathol. 2015 Oct 24;10(1):192. doi: 10.1186/s13000-015-0424-8.
26498278
Angiopoietin-like 4 enhances metastasis and inhibits apoptosis via inducing bone morphogenetic protein 7 in colorectal cancer cells.
Author : Li X1, Chen T1, Shi Q1, Li J1, Cai S1, Zhou P1, Zhong Y2, Yao L3.
Biochem Biophys Res Commun. 2015 Sep 28. pii: S0006-291X(15)30621-5. doi: 10.1016/j.bbrc.2015.09.104. [Epub ahead of print]
26417691
Elevated kinesin family member 26B is a prognostic biomarker and a potential therapeutic target for colorectal cancer.
Author : Wang J, Cui F, Wang X, Xue Y, Chen J, Yu Y, Lu H, Zhang M, Tang H, Peng Z.
J Exp Clin Cancer Res. 2015 Feb 5;34(1):13. [Epub ahead of print]
25652119
Oxidized low-density lipoprotein is associated with advanced-stage prostate cancer.
Author : Wan F1, Qin X, Zhang G, Lu X, Zhu Y, Zhang H, Dai B, Shi G, Ye D.
Tumour Biol. 2015 Jan 8. [Epub ahead of print]
25566960
Cell killing and radiosensitizing effects of atorvastatin in PC3 prostate cancer cells.
Author : He Z1, Mangala LS, Theriot CA, Rohde LH, Wu H, Zhang Y.
J Radiat Res. 2012;53(2):225-33.
22510595
MicroRNAs are involved in erythroid differentiation control.
Author : Yang GH, Wang F, Yu J, Wang XS, Yuan JY, Zhang JW.
J Cell Biochem. 2009 Jun 1;107(3):548-56. doi: 10.1002/jcb.22156.
19350553

BACKGROUND

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

REFERENCES

Azam,S., J. Biol. Chem. 283 (45), 30632-30641 (2008)
Lu,J., Biosci. Biotechnol. Biochem. 72 (9), 2432-2435 (2008)
Zhou,Y., Mol. Cancer Res. 6 (8), 1375-1384 (2008)

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