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MUC20 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 59 - MUC20 Antibody (C-term) AP7830b
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using AP7830B performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - MUC20 Antibody (C-term) AP7830b
    Anti-MUC20 Antibody (C-term) at 1:1000 dilution + Human lung tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 72 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - MUC20 Antibody (C-term) AP7830b
    Confocal immunofluorescent analysis of MUC20 Antibody (C-term)(Cat#AP7830b) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
  • 4 - MUC20 Antibody (C-term) AP7830b
    MUC20 Antibody (C-term) (Cat. #AP7830b) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, IF, FC, WB, E
Primary Accession Q8N307
Other Accession Q86ST8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 200958
Other Names Mucin-20, MUC-20, MUC20, KIAA1359
Target/Specificity This MUC20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 654-684 amino acids from the C-terminal region of human MUC20.
Dilution IHC-P-Leica~~1:500
WB~~1:1000
IF~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMUC20 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MUC20
Synonyms KIAA1359
Function May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression.
Cellular Location Secreted. Apical cell membrane. Basolateral cell membrane. Cell projection, microvillus membrane
Tissue Location Highly expressed in kidney, moderately in placenta, lung, prostate, liver, and digestive system. In the kidney, localized in the proximal tubules but not in the glomerulus or distal tubules. Detected in most of the male urogenital tract epithelia, with the exception of epididymis
Research Areas
Elaidic Acid, a Trans-Fatty Acid, Enhances the Metastasis of Colorectal Cancer Cells.
Author : Ohmori H1,Fujii K,Kadochi Y,Mori S,Nishiguchi Y,Fujiwara R,Kishi S,Sasaki T,Kuniyasu H.
Pathobiology. 2016 Nov 11. [Epub ahead of print]
27832659
Differential effect of rebamipide on transmembrane mucin biosynthesis in stratified ocular surface epithelial cells.
Author : Uchino Y1, Woodward AM1, Argyeso P2.
Exp Eye Res. 2016 Oct 7. pii: S0014-4835(16)30324-4. doi: 10.1016/j.exer.2016.10.007. [Epub ahead of print]
27725198
Expression analysis of the transmembrane mucin MUC20 in human corneal and conjunctival epithelia.
Author : Woodward AM1, Argüeso P1.
Invest Ophthalmol Vis Sci. 2014 Aug 28;55(10):6132-8. doi: 10.1167/iovs.14-15269.
25168902

BACKGROUND

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The shorter isoform expressed by this gene is localized to the plasma membrane, whereas the longer isoform might be secreted. The C terminus of this protein associates with the multifunctional docking site of the met proto-oncogene and suppresses activation of some downstream met signaling cascades. The protein features a tandem repeat domain that varies between 2 and 6 copies in different individuals. Multiple transcript variants encoding different isoforms have been found for this gene.

REFERENCES

Li,G., Am. J. Nephrol. 26 (1), 43-49 (2006)
Suzuki,Y., Genome Res. 14 (9), 1711-1718 (2004)
Higuchi,T., Mol. Cell. Biol. 24 (17), 7456-7468 (2004)
Higuchi,T., J. Biol. Chem. 279 (3), 1968-1979 (2004)

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