- 文献引用 : 2
|Application ||WB, IF, E|
|Calculated MW||71982 Da|
|Other Names||Mucin-20, MUC-20, MUC20, KIAA1359|
|Target/Specificity||This MUC20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from the N-terminal region of human MUC20.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MUC20 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression.|
|Cellular Location||Secreted. Apical cell membrane. Basolateral cell membrane. Cell projection, microvillus membrane|
|Tissue Location||Highly expressed in kidney, moderately in placenta, lung, prostate, liver, and digestive system. In the kidney, localized in the proximal tubules but not in the glomerulus or distal tubules. Detected in most of the male urogenital tract epithelia, with the exception of epididymis|
Author : Wieben ED1, Aleff RA2, Eckloff BW3, Atkinson EJ4, Baheti S4, Middha S4, Brown WL5, Patel SV5, Kocher JP4, Baratz KH5.
Invest Ophthalmol Vis Sci. 2014 Aug 28;55(9):6101-7. doi: 10.1167/iovs.14-14958.
Author : Woodward AM1, Argyeso P1.
Invest Ophthalmol Vis Sci. 2014 Aug 28;55(10):6132-8. doi: 10.1167/iovs.14-15269.
Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The shorter isoform expressed by this gene is localized to the plasma membrane, whereas the longer isoform might be secreted. The C terminus of this protein associates with the multifunctional docking site of the met proto-oncogene and suppresses activation of some downstream met signaling cascades. The protein features a tandem repeat domain that varies between 2 and 6 copies in different individuals. Multiple transcript variants encoding different isoforms have been found for this gene.
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Higuchi,T., Mol. Cell. Biol. 24 (17), 7456-7468 (2004)
Higuchi,T., J. Biol. Chem. 279 (3), 1968-1979 (2004)