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MUC20 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - MUC20 Antibody (N-term) AP7830a
    All lanes : Anti-MUC20 Antibody (N-term) at 1:2000 dilution Lane 1: Human kidney whole lysate Lane 2: Human liver whole lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 72 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - MUC20 Antibody (N-term) AP7830a
    Confocal immunofluorescent analysis of MUC20 Antibody (N-term)(Cat#AP7830a) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, E
Primary Accession Q8N307
Other Accession Q86ST8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 71982 Da
Additional info
Gene ID 200958
Other Names Mucin-20, MUC-20, MUC20, KIAA1359
Target/Specificity This MUC20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from the N-terminal region of human MUC20.
Dilution WB~~1:2000
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMUC20 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MUC20
Synonyms KIAA1359
Function May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression.
Cellular Location Secreted. Apical cell membrane. Basolateral cell membrane. Cell projection, microvillus membrane
Tissue Location Highly expressed in kidney, moderately in placenta, lung, prostate, liver, and digestive system. In the kidney, localized in the proximal tubules but not in the glomerulus or distal tubules. Detected in most of the male urogenital tract epithelia, with the exception of epididymis
Research Areas
Comprehensive assessment of genetic variants within TCF4 in Fuchs\' endothelial corneal dystrophy.
Author : Wieben ED1, Aleff RA2, Eckloff BW3, Atkinson EJ4, Baheti S4, Middha S4, Brown WL5, Patel SV5, Kocher JP4, Baratz KH5.
Invest Ophthalmol Vis Sci. 2014 Aug 28;55(9):6101-7. doi: 10.1167/iovs.14-14958.
25168903
Expression analysis of the transmembrane mucin MUC20 in human corneal and conjunctival epithelia.
Author : Woodward AM1, Argyeso P1.
Invest Ophthalmol Vis Sci. 2014 Aug 28;55(10):6132-8. doi: 10.1167/iovs.14-15269.
25168902

BACKGROUND

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The shorter isoform expressed by this gene is localized to the plasma membrane, whereas the longer isoform might be secreted. The C terminus of this protein associates with the multifunctional docking site of the met proto-oncogene and suppresses activation of some downstream met signaling cascades. The protein features a tandem repeat domain that varies between 2 and 6 copies in different individuals. Multiple transcript variants encoding different isoforms have been found for this gene.

REFERENCES

Li,G., Am. J. Nephrol. 26 (1), 43-49 (2006)
Suzuki,Y., Genome Res. 14 (9), 1711-1718 (2004)
Higuchi,T., Mol. Cell. Biol. 24 (17), 7456-7468 (2004)
Higuchi,T., J. Biol. Chem. 279 (3), 1968-1979 (2004)

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