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TGF Beta Receptor I Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - TGF Beta Receptor I Antibody (Center) AP7822c
    Western blot analysis of anti-TGFBR1 Pab (Cat. #AP7822c) in Jurkat cell line lysates (35ug/lane). TGFBR1 (arrow) was detected using the purified Pab.
  • 14 - TGF Beta Receptor I Antibody (Center) AP7822c
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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  • 文献引用 : 6
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P36897
Other Accession P80204, Q5CD18, Q64729, O46680
Reactivity Human, Mouse
Predicted Rat, Pig, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 7046
Other Names TGF-beta receptor type-1, TGFR-1, Activin A receptor type II-like protein kinase of 53kD, Activin receptor-like kinase 5, ALK-5, ALK5, Serine/threonine-protein kinase receptor R4, SKR4, TGF-beta type I receptor, Transforming growth factor-beta receptor type I, TGF-beta receptor type I, TbetaR-I, TGFBR1, ALK5, SKR4
Target/Specificity This TGF Beta Receptor I antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-163 amino acids from the Central region of human TGF Beta Receptor I.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTGF Beta Receptor I Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TGFBR1
Synonyms ALK5, SKR4
Function Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non- canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation.
Cellular Location Cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Cell surface. Membrane raft
Tissue Location Found in all tissues examined, most abundant in placenta and least abundant in brain and heart. Expressed in a variety of cancer cell lines (PubMed:25893292)
Research Areas
Effects of secreted frizzled-related protein 1 on proliferation, migration, invasion, and apoptosis of colorectal cancer cells.
Author : Wang Z#1,Li R#1,He Y1,Huang S1.
Cancer Cell Int. 2018 Mar 27;18:48. doi: 10.1186/s12935-018-0543-x. eCollection 2018.
29610564
miR-22 regulates C2C12 myoblast proliferation and differentiation by targeting TGFBR1.
Author : Wang H1,Zhang Q2,Wang B2,Wu W2,Wei J2,Li P3,Huang R4.
Eur J Cell Biol. 2018 Mar 21. pii: S0171-9335(17)30316-3. doi: 10.1016/j.ejcb.2018.03.006. [Epub ahead of print]
29588073
Effect of astragalus injection on renal tubular epithelial transdifferentiation in type 2 diabetic mice.
Author : Yi YE1,Li SY1,Nie YN1,Jia DX1,Zhang ZH1,Wang YF1,Wang Q2.
BMC Complement Altern Med. 2016 Jul 16;16:222. doi: 10.1186/s12906-016-1208-8.
27422712
miR-140-5p regulates adipocyte differentiation by targeting transforming growth factor-β signaling.
Author : Zhang X1, Chang A2, Li Y1, Gao Y1, Wang H3, Ma Z2, Li X4, Wang B1.
Sci Rep. 2015 Dec 11;5:18118. doi: 10.1038/srep18118.
26657345
CD36 is involved in high glucose-induced epithelial to mesenchymal transition in renal tubular epithelial cells.
Author : Hou Y1, Wu M2, Wei J3, Ren Y3, Du C3, Wu H3, Li Y4, Shi Y5.
Biochem Biophys Res Commun. 2015 Oct 24. pii: S0006-291X(15)30812-3. doi: 10.1016/j.bbrc.2015.10.112. [Epub ahead of print]
26505798
Increased TGF-β1-mediated suppression of growth and motility in castrate-resistant prostate cancer cells is consistent with Smad2/3 signaling.
Author : Miles FL, Tung NS, Aguiar AA, Kurtoglu S, Sikes RA.
Prostate. 2012 Sep 1;72(12):1339-50. doi: 10.1002/pros.22482. Epub 2012 Jan 6.
22228025

BACKGROUND

The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS).

REFERENCES

Itoh, S., et al., J. Biol. Chem. 278(6):3751-3761 (2003). Valcourt, U., et al., J. Biol. Chem. 277(37):33545-33558 (2002). Bourguignon, L.Y., et al., J. Biol. Chem. 277(42):39703-39712 (2002). Jude, E.B., et al., Diabet. Med. 19(6):440-447 (2002). Nagel, D., et al., Biochem. Biophys. Res. Commun. 290(5):1558-1563 (2002).

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