注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   神经科学   >   NTF3 Antibody (C-term)   

NTF3 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - NTF3 Antibody (C-term) AP7763b
    Overlay histogram showing Hela cells stained with AP7763B(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP7763B, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - NTF3 Antibody (C-term) AP7763b
    All lanes : Anti-NTF3 Antibody (C-term) at 1:2000 dilution Lane 1: Human brain lysate Lane 2: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - NTF3 Antibody (C-term) AP7763b
    All lanes : Anti-NTF3 Antibody (C-term) at 1:2000 dilution Lane 1: Human ovary lysate Lane 2: Human spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - NTF3 Antibody (C-term) AP7763b
    Anti-NTF3 Antibody (C-term) at 1:2000 dilution + NTF3 protein whole cell lysate Lysates/proteins at 20 ng per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 13. 5 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - NTF3 Antibody (C-term) AP7763b
    All lanes : Anti-NTF3 Antibody (C-term) at 1:2000 dilution Lane 1: Human liver lysate Lane 2: Human ovary lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession P20783
Other Accession P25435, P18280, Q06AV0, P20181, P25433, Q08DT3
Reactivity Human, Mouse, Rat
Predicted Rat, Pig, Chicken, Xenopus, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 4908
Other Names Neurotrophin-3, NT-3, HDNF, Nerve growth factor 2, NGF-2, Neurotrophic factor, NTF3
Target/Specificity This NTF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-184 amino acids from the C-terminal region of human NTF3.
Dilution FC~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNTF3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NTF3
Function Seems to promote the survival of visceral and proprioceptive sensory neurons.
Cellular Location Secreted.
Tissue Location Brain and peripheral tissues.
Research Areas

BACKGROUND

NTF3 is a member of the neurotrophin family that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.

REFERENCES

Hossain,W.A., J. Neurosci. Res. 86 (11), 2376-2391 (2008)
Mercader,J.M., Hum. Mol. Genet. 17 (9), 1234-1244 (2008)
Kalcheim,C.,Proc. Natl. Acad. Sci. U.S.A. 89 (5), 1661-1665 (1992)

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价