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APOA4 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - APOA4 Antibody (N-term) AP7591a
    Western blot analysis of APOA4 Antibody (N-term) (Cat.#AP7591a) in Jurkat, K562, NCI-H460 cell line lysates (35ug/lane). APOA4 (arrow) was detected using the purified Pab.
  • 14 - APOA4 Antibody (N-term) AP7591a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APOA4 antibody (N-term) (Cat.#AP7591a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P06727
Other Accession P33621, A8MSL6
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 45399 Da
Additional info
Other Names Apolipoprotein A-IV, Apo-AIV, ApoA-IV, Apolipoprotein A4, APOA4
Target/Specificity This APOA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-79 amino acids from the N-terminal region of human APOA4.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAPOA4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name APOA4
Function May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
Cellular Location Secreted.
Tissue Location Synthesized primarily in the intestine and secreted in plasma
Research Areas

BACKGROUND

The primary translation product of the APOA4 gene is a 396-residue preprotein which after proteolytic processing is secreted into its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

REFERENCES

Chien,K.L., Clin. Chim. Acta 388 (1-2), 78-83 (2008)
Bai,H., Acta Cardiol 63 (3), 315-322 (2008)

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