|Application ||WB, IHC-P, E|
|Other Accession||P33621, A8MSL6|
|Calculated MW||45399 Da|
|Other Names||Apolipoprotein A-IV, Apo-AIV, ApoA-IV, Apolipoprotein A4, APOA4|
|Target/Specificity||This APOA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-79 amino acids from the N-terminal region of human APOA4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||APOA4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.|
|Tissue Location||Synthesized primarily in the intestine and secreted in plasma|
Provided below are standard protocols that you may find useful for product applications.
The primary translation product of the APOA4 gene is a 396-residue preprotein which after proteolytic processing is secreted into its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.
Chien,K.L., Clin. Chim. Acta 388 (1-2), 78-83 (2008)
Bai,H., Acta Cardiol 63 (3), 315-322 (2008)