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PFTK1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PFTK1 Antibody (N-term) AP7550a
    Western blot analysis of lysate from human brain tissue lysate, using PFTK1 Antibody (N-term )(Cat. #AP7550A). AP7550A was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • 1 - PFTK1 Antibody (N-term) AP7550a
    Western blot analysis of lysates from Hela cell line , human brain and mouse cerebellum tissue lysate(from left to right), using PFTK1 Antibody (N-term )(Cat. #AP7550A). AP7550A was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • 1 - PFTK1 Antibody (N-term) AP7550a
    Western blot analysis of PFTK1 antibody (N-term) (Cat.#AP7550a) in mouse cerebellum tissue lysates (35ug/lane). PFTK1 (arrow) was detected using the purified Pab.
  • 14 - PFTK1 Antibody (N-term) AP7550a
    Formalin-fixed and paraffin-embedded human testis tissue reacted with PFTK1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession O94921
Other Accession B6A7Q3, O35495, NP_036527
Reactivity Human, Mouse
Predicted Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 53057 Da
Additional info
Gene ID 5218
Other Names Cyclin-dependent kinase 14, Cell division protein kinase 14, Serine/threonine-protein kinase PFTAIRE-1, hPFTAIRE1, CDK14, KIAA0834, PFTK1
Target/Specificity This PFTK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PFTK1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPFTK1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDK14
Synonyms KIAA0834, PFTK1
Function Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport.
Cellular Location Cell membrane; Peripheral membrane protein. Cytoplasm. Nucleus. Note=Recruited to the cell membrane by CCNY
Tissue Location Highly expressed in brain, pancreas, kidney, heart, testis and ovary. Also detected at lower levels in other tissues except in spleen and thymus where expression is barely detected.
Research Areas

BACKGROUND

PFTK1, a member of the CDC2/CDKX subfamily of Ser/Thr protein kinases, may play a role in meiosis as well as in neuron differentiation and/or function It is highly expressed in brain, pancreas, kidney, heart, testis and ovary, and also detected at lower levels in other tissues except in spleen and thymus where expression is minimal.

REFERENCES

Yang, T., et al., Gene 267(2):165-172 (2001).
Nagase, T., et al., DNA Res. 5(6):355-364 (1998).

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