- 文献引用 : 1
|Application ||WB, IHC-P, E|
|Other Accession||B6A7Q3, O35495, NP_036527|
|Calculated MW||53057 Da|
|Other Names||Cyclin-dependent kinase 14, Cell division protein kinase 14, Serine/threonine-protein kinase PFTAIRE-1, hPFTAIRE1, CDK14, KIAA0834, PFTK1|
|Target/Specificity||This PFTK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PFTK1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PFTK1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport.|
|Cellular Location||Cell membrane; Peripheral membrane protein. Cytoplasm. Nucleus. Note=Recruited to the cell membrane by CCNY|
|Tissue Location||Highly expressed in brain, pancreas, kidney, heart, testis and ovary. Also detected at lower levels in other tissues except in spleen and thymus where expression is barely detected.|
Author : Wang X1,Jia Y1,Fei C1,Song X2,Li L3.
J Biol Chem. 2016 Dec 16;291(51):26427-26434. Epub 2016 Nov 7.
Provided below are standard protocols that you may find useful for product applications.
PFTK1, a member of the CDC2/CDKX subfamily of Ser/Thr protein kinases, may play a role in meiosis as well as in neuron differentiation and/or function It is highly expressed in brain, pancreas, kidney, heart, testis and ovary, and also detected at lower levels in other tissues except in spleen and thymus where expression is minimal.
Yang, T., et al., Gene 267(2):165-172 (2001).
Nagase, T., et al., DNA Res. 5(6):355-364 (1998).