- 文献引用 : 3
|Application ||WB, IHC-P, E|
|Other Accession||O88850, Q9ERH7|
|Reactivity||Human, Mouse, Rat|
|Other Names||Homeodomain-interacting protein kinase 3, Androgen receptor-interacting nuclear protein kinase, ANPK, Fas-interacting serine/threonine-protein kinase, FIST, Homolog of protein kinase YAK1, HIPK3, DYRK6, FIST3, PKY|
|Target/Specificity||This HIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1127-1156 amino acids from the C-terminal region of human HIPK3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HIPK3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||DYRK6, FIST3, PKY|
|Function||Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF- responsive element (OCFRE).|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Overexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung.|
Author : Lan HC1, Wu CF, Shih HM, Chung BC.
J Biol Chem. 2012 Feb 17;287(8):5910-6. doi: 10.1074/jbc.M111.307603. Epub 2011 Dec 24.
Author : Tiefenbach J1, Moll PR, Nelson MR, Hu C, Baev L, Kislinger T, Krause HM.
PLoS One. 2010 Mar 22;5(3):e9797. doi: 10.1371/journal.pone.0009797.
Author : Lan HC, Li HJ, Lin G, Lai PY, Chung BC.
Mol Cell Biol. 2007 Mar;27(6):2027-36. Epub 2007 Jan 8.
Provided below are standard protocols that you may find useful for product applications.
HIPK3 negatively regulates apoptosis by promoting FADD phosphorylation. This kinase enhances androgen receptor-mediated transcription, and may act as a transcriptional corepressor for NK homeodomain transcription factors.
Blume-Jensen P, et al. Nature 2001. 411: 355.
Cantrell D, J. Cell Sci. 2001. 114: 1439.
Jhiang S Oncogene 2000. 19: 5590.
Manning G, et al. Science 2002. 298: 1912.
Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359.
Robertson, S. et al. Trends Genet. 2000. 16: 368.
Robinson D, et al. Oncogene 2000. 19: 5548.
Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889.
Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561.
Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.