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DYRK2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - DYRK2 Antibody (N-term) AP7534a
    Western blot analysis of lysates from CEM, Hela cell line (from left to right), using DYRK2 Antibody (P46) (Cat. #AP7534a). AP7534a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • 1 - DYRK2 Antibody (N-term) AP7534a
    The anti-DYRK2 Pab (Cat. #AP7534a) is used in Western blot to detect DYRK2 in 293 cell lysate.
  • 14 - DYRK2 Antibody (N-term) AP7534a
    DYRK2 Antibody (N-term) (Cat. #AP7534A)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DYRK2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q92630
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 8445
Other Names Dual specificity tyrosine-phosphorylation-regulated kinase 2, DYRK2
Target/Specificity This DYRK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-135 amino acids from the N-terminal region of human DYRK2.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDYRK2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DYRK2
Function Serine/threonine-protein kinase involved in the regulation of the mitotic cell cycle, cell proliferation, apoptosis, organization of the cytoskeleton and neurite outgrowth. Functions in part via its role in ubiquitin-dependent proteasomal protein degradation. Functions downstream of ATM and phosphorylates p53/TP53 at 'Ser-46', and thereby contributes to the induction of apoptosis in response to DNA damage. Phosphorylates NFATC1, and thereby inhibits its accumulation in the nucleus and its transcription factor activity. Phosphorylates EIF2B5 at 'Ser-544', enabling its subsequent phosphorylation and inhibition by GSK3B. Likewise, phosphorylation of NFATC1, CRMP2/DPYSL2 and CRMP4/DPYSL3 promotes their subsequent phosphorylation by GSK3B. May play a general role in the priming of GSK3 substrates. Inactivates GYS1 by phosphorylation at 'Ser- 641', and potentially also a second phosphorylation site, thus regulating glycogen synthesis. Mediates EDVP E3 ligase complex formation and is required for the phosphorylation and subsequent degradation of KATNA1. Phosphorylates TERT at 'Ser-457', promoting TERT ubiquitination by the EDVP complex. Phosphorylates SIAH2, and thereby increases its ubiquitin ligase activity. Promotes the proteasomal degradation of MYC and JUN, and thereby regulates progress through the mitotic cell cycle and cell proliferation. Promotes proteasomal degradation of GLI2 and GLI3, and thereby plays a role in smoothened and sonic hedgehog signaling. Plays a role in cytoskeleton organization and neurite outgrowth via its phosphorylation of DCX and DPYSL2. Phosphorylates CRMP2/DPYSL2, CRMP4/DPYSL3, DCX, EIF2B5, EIF4EBP1, GLI2, GLI3, GYS1, JUN, MDM2, MYC, NFATC1, p53/TP53, TAU/MAPT and KATNA1. Can phosphorylate histone H1, histone H3 and histone H2B (in vitro). Can phosphorylate CARHSP1 (in vitro).
Cellular Location Cytoplasm. Nucleus. Note=Translocates into the nucleus following DNA damage
Tissue Location Testis, after the onset of spermatogenesis.
Research Areas
Downregulation of dual-specificity tyrosine-regulated kinase 2 promotes tumor cell proliferation and invasion by enhancing cyclin-dependent kinase 14 expression in breast cancer.
Author : Imawari Y1,2,Mimoto R2,Hirooka S3,Morikawa T2,Takeyama H2,Yoshida K1.
Cancer Sci. 2018 Feb;109(2):363-372. doi: 10.1111/cas.13459. Epub 2018 Jan 13.
29193658
Impairment of DYRK2 augments stem-like traits by promoting KLF4 expression in breast cancer.
Author : Mimoto R1,2, Imawari Y1,2, Hirooka S3, Takeyama H2, Yoshida K1.
Oncogene. 2016 Oct 10. doi: 10.1038/onc.2016.349. [Epub ahead of print]
27721402
The Expression of the Ubiquitin Ligase SIAH2 (Seven In Absentia Homolog 2) Is Increased in Human Lung Cancer.
Author : Moreno P1,2, Lara-Chica M1, Soler-Torronteras R1, Caro T3, Medina M3, Álvarez A2, Salvatierra Á2, Muñoz E1, Calzado MA1.
PLoS One. 2015 Nov 18;10(11):e0143376. doi: 10.1371/journal.pone.0143376. eCollection 2015.
26580787
Dual-specificity tyrosine phosphorylation-regulated kinase 2 (DYRK2) as a novel marker in T1 high-grade and T2 bladder cancer patients receiving neoadjuvant chemotherapy : DYRK2 is associated with survival in bladder cancer.
Author : Nomura S1, Suzuki Y2, Takahashi R3, Terasaki M4, Kimata R5, Terasaki Y6, Hamasaki T7, Kimura G8, Shimizu A9, Kondo Y10.
BMC Urol. 2015 Jun 19;15:53. doi: 10.1186/s12894-015-0040-7.
26087959
Downregulation of DYRK2 can be a predictor of recurrence in early stage breast cancer.
Author : Enomoto Y1, Yamashita SI, Yoshinaga Y, Fukami Y, Miyahara S, Nabeshima K, Iwasaki A.
Tumour Biol. 2014 Aug 6. [Epub ahead of print]
25095982
Mutual regulation between SIAH2 and DYRK2 controls hypoxic and genotoxic signaling pathways.
Author : Pérez M, García-Limones C, Zapico I, Marina A, Schmitz ML, Muñoz E, Calzado MA.
J Mol Cell Biol. 2012 Oct;4(5):316-30. doi: 10.1093/jmcb/mjs047. Epub 2012 Aug 9.
22878263
DYRK2 priming phosphorylation of c-Jun and c-Myc modulates cell cycle progression in human cancer cells.
Author : Taira N, Mimoto R, Kurata M, Yamaguchi T, Kitagawa M, Miki Y, Yoshida K.
J Clin Invest. 2012 Mar 1;122(3):859-72. doi: 10.1172/JCI60818. Epub 2012 Feb 6.
22307329
Expression of dual-specificity tyrosine-(Y)-phosphorylation-regulated kinase 2 (DYRK2) can be a favorable prognostic marker in pulmonary adenocarcinoma.
Author : Yamashita S, Chujo M, Tokuishi K, Anami K, Miyawaki M, Yamamoto S, Kawahara K.
J Thorac Cardiovasc Surg. 2009 Dec;138(6):1303-8. doi: 10.1016/j.jtcvs.2009.08.003. Epub 2009 Oct 9.
19818968
DYRK2 expression may be a predictive marker for chemotherapy in non-small cell lung cancer.
Author : Yamashita S, Chujo M, Moroga T, Anami K, Tokuishi K, Miyawaki M, Kawano Y, Takeno S, Yamamoto S, Kawahara K.
Anticancer Res. 2009 Jul;29(7):2753-7.
19596956

BACKGROUND

DYRK2 belongs to a family of protein kinases whose members are presumed to be involved in cellular growth and/or development. The family is defined by structural similarity of their kinase domains and their capability to autophosphorylate on tyrosine residues. DYRK2 has demonstrated tyrosine autophosphorylation and catalyzed phosphorylation of histones H3 and H2B in vitro.

REFERENCES

Becker, W., et al., J. Biol. Chem. 273(40):25893-25902 (1998).

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