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EN1 (Engrailed 1) Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - EN1 (Engrailed 1) Antibody (N-term) AP7278a
    All lanes : Anti-EN1 Antibody (N-term) at 1:2000 dilution Lane 1: U-2OS whole cell lysate Lane 2: U87-MG whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lane 4: 293T/17 whole cell lysate Lane 5: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - EN1 (Engrailed 1) Antibody (N-term) AP7278a
    All lanes : Anti-EN1 Antibody (N-term) at 1:1000 dilution Lane 1: U-2OS whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P, E
Primary Accession Q05925
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 2019
Other Names Homeobox protein engrailed-1, Homeobox protein en-1, Hu-En-1, EN1
Target/Specificity This EN1 (Engrailed 1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EN1 (Engrailed 1).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEN1 (Engrailed 1) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EN1
Cellular Location Nucleus.
Research Areas
Differential Neuronal Plasticity of Dental Pulp Stem Cells From Exfoliated Deciduous and Permanent Teeth Towards Dopaminergic Neurons.
Author : Majumdar D1,Kanafi M1,Bhonde R1,Gupta P2,Datta I3.
J Cell Physiol. 2016 Sep;231(9):2048-63. doi: 10.1002/jcp.25314. Epub 2016 Feb 4.
26773559

BACKGROUND

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

REFERENCES

Bachar-Dahan,L., Mol. Biol. Cell 17 (6), 2572-2580 (2006)
Kohler,A., Genomics 15 (1), 233-235 (1993)

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