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|Application ||WB, IF, IHC-P, E|
|Other Names||Homeobox protein engrailed-1, Homeobox protein en-1, Hu-En-1, EN1|
|Target/Specificity||This EN1 (Engrailed 1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EN1 (Engrailed 1).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EN1 (Engrailed 1) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
Author : Majumdar D1,Kanafi M1,Bhonde R1,Gupta P2,Datta I3.
J Cell Physiol. 2016 Sep;231(9):2048-63. doi: 10.1002/jcp.25314. Epub 2016 Feb 4.
Provided below are standard protocols that you may find useful for product applications.
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.
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