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|Application ||WB, IHC-P, E|
|Calculated MW||64987 Da|
|Other Names||M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B, CDC25B, CDC25HU2|
|Target/Specificity||This CDC25B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-360 amino acids from the Central region of human CDC25B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CDC25B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.|
|Cellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole|
Author : Hsieh WL1, Huang YH, Wang TM, Ming YC, Tsai CN, Pang JH.
Cell Prolif. 2015 Feb 9. doi: 10.1111/cpr.12168. [Epub ahead of print]
Provided below are standard protocols that you may find useful for product applications.
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.
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