- 文献引用 : 2
|Application ||WB, IHC-P, E|
|Calculated MW||69217 Da|
|Other Names||Sphingosine kinase 2, SK 2, SPK 2, SPHK2|
|Target/Specificity||This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 590-620 amino acids from the C-terminal region of human SPHK2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPHK2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro- dihydrosphingosine, D-erythro-sphingosine and L-threo- dihydrosphingosine. Binds phosphoinositides.|
|Cellular Location||Isoform 1: Cytoplasm. Membrane.|
Author : Sun E1, Zhang W, Wang L, Wang A, Ma C, Lei M, Zhou X, Sun Y, Lu B, Liu L, Han R.
Tumour Biol. 2015 Jul 31. [Epub ahead of print]
Author : Coste O1, Brenneis C, Linke B, Pierre S, Maeurer C, Becker W, Schmidt H, Gao W, Geisslinger G, Scholich K.
J Biol Chem. 2008 Nov 21;283(47):32442-51. doi: 10.1074/jbc.M806410200. Epub 2008 Sep 19.
Provided below are standard protocols that you may find useful for product applications.
Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.
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