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SPHK2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - SPHK2 Antibody (C-term) AP7238b
    All lanes : Anti-hSPHK2-A605 at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: 293 whole cell lysate Lane 4: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - SPHK2 Antibody (C-term) AP7238b
    SPHK2 Antibody (A605) (Cat. #AP7238b) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SPHK2 antibody detected the SPHK2 protein (arrow).
  • 14 - SPHK2 Antibody (C-term) AP7238b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9NRA0
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 69217 Da
Additional info
Gene ID 56848
Other Names Sphingosine kinase 2, SK 2, SPK 2, SPHK2
Target/Specificity This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 590-620 amino acids from the C-terminal region of human SPHK2.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPHK2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPHK2
Function Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro- dihydrosphingosine, D-erythro-sphingosine and L-threo- dihydrosphingosine. Binds phosphoinositides.
Cellular Location Isoform 1: Cytoplasm. Membrane.
Research Areas
Down-regulation of Sphk2 suppresses bladder cancer progression.
Author : Sun E1, Zhang W, Wang L, Wang A, Ma C, Lei M, Zhou X, Sun Y, Lu B, Liu L, Han R.
Tumour Biol. 2015 Jul 31. [Epub ahead of print]
26224479
Sphingosine 1-phosphate modulates spinal nociceptive processing.
Author : Coste O1, Brenneis C, Linke B, Pierre S, Maeurer C, Becker W, Schmidt H, Gao W, Geisslinger G, Scholich K.
J Biol Chem. 2008 Nov 21;283(47):32442-51. doi: 10.1074/jbc.M806410200. Epub 2008 Sep 19.
18805787

BACKGROUND

Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

REFERENCES

Shu, X., et al., Mol. Cell. Biol. 22(22):7758-7768 (2002).
Xia, P., et al., J. Biol. Chem. 277(10):7996-8003 (2002).
Liu, H., et al., J. Biol. Chem. 275(26):19513-19520 (2000).
Shutler, G., et al., Genomics 34(3):334-339 (1996).

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