注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   神经科学   >   DCAMKL1 Antibody (C-term)   

DCAMKL1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
标注"皇冠"的产品,是满足高质量标准的产品。
了解更多...
  • 1 - DCAMKL1 Antibody (C-term) AP7219b
    All lanes : Anti-hDCAMKL1-V705 at 1:8000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lane 3: Rat brain lysate Lane 4: TT whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - DCAMKL1 Antibody (C-term) AP7219b
    Expression pattern of doublecortin and CaM kinase-like-1 (DCAMKL-1) in normal mouse small intestine. (A): Immunohistochemical staining of normal small intestine for DCAMKL-1. Arrows indicate the cells positive for DCAMKL-1 in the stem cell zone. (B): Preincubation with blocking peptide completely abolished DCAMKL-1 immunoreactivity. (C): Immunohistochemical staining of normal small intestine for DCAMKL-1. Brown indicates the cells positive for DCAMKL-1 (indicated by the arrows).
  • 3 - DCAMKL1 Antibody (C-term) AP7219b
    (C): The cell positive for DCAMKL-1, stained red (indicated by the arrow), appears at the base of the crypt. (D): Intestinal section stained for Musashi-1 (green). (E): Colocalization of DCAMKL-1 and Musashi-1 (yellow, indicated by the arrow). The magnified inset image represents the single cell positive for both DCAMKL-1 and Musashi-1. (F): Costaining of DCMAKL-1 (red, indicated by the arrow) with nuclear Hoechst 33342 (blue) staining. (G): Costaining of Musashi-1 (green) with nuclear Hoechst 33342 (blue) staining. (H): Colocalization of DCAMKL-1 and Musashi-1 (yellow, indicated by the arrow), costained with nuclear Hoechst 33342 (blue) staining. The magnified inset image represents the single cell positive for both DCAMKL-1 and Musashi-1 (yellow).
  • 产品详情
  • 文献引用 : 18
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, E
Primary Accession O15075
Other Accession Q9JLM8
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 82224 Da
Additional info
Gene ID 9201
Other Names Serine/threonine-protein kinase DCLK1, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1, Doublecortin-like kinase 1, DCLK1, DCAMKL1, DCDC3A, KIAA0369
Target/Specificity This DCAMKL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 690-720 amino acids from the C-terminal region of human DCAMKL1.
Dilution WB~~1:8000
IHC-P~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDCAMKL1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DCLK1
Synonyms DCAMKL1, DCDC3A, KIAA0369
Function Probable kinase that may be involved in a calcium- signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
Tissue Location In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain.
Research Areas
Association of doublecortin-like kinase 1 with tumor aggressiveness and poor biochemical recurrence-free survival in prostate cancer.
Author : Jiang D#1,Xiao C#1,Xian T1,Wang L2,Mao Y1,Zhang J1,Pang J1,3.
Onco Targets Ther. 2018 Feb 28;11:1077-1086. doi: 10.2147/OTT.S157295. eCollection 2018.
29535532
Organoid Cultures for Assessing Intestinal Epithelial Differentiation and Function in Response to Type-2 Inflammation.
Author : Zwarycz B1,Gracz AD2,Magness ST3,4,5.
Methods Mol Biol. 2018;1799:397-417. doi: 10.1007/978-1-4939-7896-0_29.
29956167
Helicobacter-induced gastric inflammation alters the properties of gastric tissue stem/progenitor cells.
Author : Shibata W1,2,Sue S1,Tsumura S1,3,Ishii Y1,Sato T1,Kameta E1,Sugimori M1,Yamada H1,Kaneko H1,Sasaki T1,Ishii T1,Tamura T1,Kondo M1,Maeda S4.
BMC Gastroenterol. 2017 Dec 6;17(1):145. doi: 10.1186/s12876-017-0706-6.
29212456
Contribution of ATOH1+ Cells to the Homeostasis, Repair, and Tumorigenesis of the Colonic Epithelium.
Author : Ishibashi F1,Shimizu H2,Nakata T1,Fujii S1,Suzuki K1,Kawamoto A1,Anzai S1,Kuno R1,Nagata S1,Ito G3,Murano T1,Mizutani T1,Oshima S1,Tsuchiya K1,Nakamura T4,Watanabe M1,Okamoto R5.
Stem Cell Reports. 2017 Dec 1. pii: S2213-6711(17)30492-7. doi: 10.1016/j.stemcr.2017.11.006. [Epub ahead of print]
29233556
Enhancement of cytotoxic effects of gemcitabine by Dclk1 inhibition through suppression of Chk1 phosphorylation in human pancreatic cancer cells.
Author : Kawamura D1,Takemoto Y1,Nishimoto A2,Ueno K1,Hosoyama T1,Shirasawa B2,Tanaka T1,Kugimiya N1,Harada E1,Hamano K1.
Oncol Rep. 2017 Sep 20. doi: 10.3892/or.2017.5974. [Epub ahead of print]
29048622
Goblet Cell Ratio in Combination with Differentiation and Stem Cell Markers in Barrett Esophagus Allow Distinction of Patients with and without Esophageal Adenocarcinoma.
Author : Schellnegger R1,Quante A2,3,Rospleszcz S4,Schernhammer M1,Höhl B1,Tobiasch M1,Pastula A1,Brandtner A1,Abrams JA5,Strauch K2,3,Schmid RM1,Vieth M6,Wang TC5,Quante M7.
Cancer Prev Res (Phila). 2017 Jan;10(1):55-66. doi: 10.1158/1940-6207.CAPR-16-0117. Epub 2016 Nov 2.
27807078
Data showing proliferation and differentiation of intestinal epithelial cells under targeted depletion of Notch ligands in mouse intestine.
Author : Nakata T1,Shimizu H2,Nagata S1,Ito G3,Fujii S1,Suzuki K1,Kawamoto A1,Ishibashi F1,Kuno R1,Anzai S1,Murano T1,Mizutani T1,Oshima S1,Tsuchiya K1,Nakamura T4,Hozumi K5,Watanabe M1,Okamoto R6.
Data Brief. 2016 Dec 29;10:551-556. doi: 10.1016/j.dib.2016.12.045. eCollection 2017.
28124021
Doublecortin-like kinase 1-positive enterocyte - a new cell type in human intestine.
Author : Leppanen J1,2,3,4, Helminen O5,6,7,8, Huhta H5,6,7,8, Kauppila JH5,6,7,8, Miina
APMIS. 2016 Sep 28. doi: 10.1111/apm.12599. [Epub ahead of print]
27677532
DCLK1 is up-regulated and associated with metastasis and prognosis in colorectal cancer.
Author : Gao T1, Wang M1, Xu L1, Wen T2, Liu J3, An G4.
J Cancer Res Clin Oncol. 2016 Aug 12. [Epub ahead of print]
27520310
Catecholamines Facilitate Fuel Expenditure and Protect Against Obesity via a Novel Network of the Gut-Brain Axis in Transcription Factor Skn-1-deficient Mice.
Author : Ushiama S1, Ishimaru Y2, Narukawa M1, Yoshioka M1, Kozuka C3, Watanabe N4, Tsunoda M5, Osakabe N4, Asakura T1, Masuzaki H3, Abe K6.
EBioMedicine. 2016 Jun;8:60-71. doi: 10.1016/j.ebiom.2016.04.031. Epub 2016 Apr 30.
27428419
Nkx2.2 is expressed in a subset of enteroendocrine cells with expanded lineage potential.
Author : Gross S, Balderes D, Liu J, Asfaha S1, Gu G2, Wang TC, Sussel L3.
Am J Physiol Gastrointest Liver Physiol. 2015 Oct 22:ajpgi.00244.2015. doi: 10.1152/ajpgi.00244.2015. [Epub ahead of print]
26492922
Cell lineage identification and stem cell culture in a porcine model for the study of intestinal epithelial regeneration.
Author : Gonzalez LM, Williamson I, Piedrahita JA, Blikslager AT, Magness ST.
PLoS One. 2013 Jun 28;8(6):e66465. doi: 10.1371/journal.pone.0066465. Print 2013.
23840480
Glucagon-like peptide-2 increases dysplasia in rodent models of colon cancer.
Author : Trivedi S, Wiber SC, El-Zimaity HM, Brubaker PL.
Am J Physiol Gastrointest Liver Physiol. 2012 Apr 15;302(8):G840-9. doi: 10.1152/ajpgi.00505.2011. Epub 2012 Feb 9.
22323126
Krüppel-like factor 4 regulates intestinal epithelial cell morphology and polarity.
Author : Yu T, Chen X, Zhang W, Li J, Xu R, Wang TC, Ai W, Liu C.
PLoS One. 2012;7(2):e32492. doi: 10.1371/journal.pone.0032492. Epub 2012 Feb 24.
22384261
Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium.
Author : Gerbe F, van Es JH, Makrini L, Brulin B, Mellitzer G, Robine S, Romagnolo B, Shroyer NF, Bourgaux JF, Pignodel C, Clevers H, Jay P.
J Cell Biol. 2011 Mar 7;192(5):767-80. doi: 10.1083/jcb.201010127.
21383077
DCAMKL-1 expression identifies Tuft cells rather than stem cells in the adult mouse intestinal epithelium.
Author : Gerbe F, Brulin B, Makrini L, Legraverend C, Jay P.
Gastroenterology. 2009 Dec;137(6):2179-80; author reply 2180-1. doi: 10.1053/j.gastro.2009.06.072. Epub 2009 Oct 29.
19879217
Regeneration of intestinal stem/progenitor cells following doxorubicin treatment of mice.
Author : Dekaney CM, Gulati AS, Garrison AP, Helmrath MA, Henning SJ.
Am J Physiol Gastrointest Liver Physiol. 2009 Sep;297(3):G461-70. doi: 10.1152/ajpgi.90446.2008. Epub 2009 Jul 9.
19589945
Identification of a novel putative gastrointestinal stem cell and adenoma stem cell marker, doublecortin and CaM kinase-like-1, following radiation injury and in adenomatous polyposis coli/multiple intestinal neoplasia mice.
Author : May R, Riehl TE, Hunt C, Sureban SM, Anant S, Houchen CW.
Stem Cells. 2008 Mar;26(3):630-7. Epub 2007 Nov 29.
18055444

BACKGROUND

Doublecortin-like kinase (DCAMKL1)(Ser/Thr protein kinase family) is essential for proper neurogenesis, neuronal migration, and axonal wiring. DCAMKL1 is involved in a calcium-signaling pathway controling neuronal migration in the developing brain, and participates in functions of the mature nervous system. DCAMKL1 protein shares high homology with doublecortin (DCX). DCLK, but not DCX, is highly expressed in regions of active neurogenesis in the neocortex and cerebellum. DCAMKL1 controls mitotic division by regulating spindle formation and also determines the fate of neural progenitors during cortical neurogenesis.

REFERENCES

Matsumoto, N., et al., Genomics 56(2):179-183 (1999). Sossey-Alaoui, K., et al., Genomics 56(1):121-126 (1999). Omori, Y., et al., J. Hum. Genet. 43(3):169-177 (1998). Nagase, T., et al., DNA Res. 4(2):141-150 (1997).

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价