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|Application ||WB, IHC-P, E|
|Other Names||Calcium/calmodulin-dependent protein kinase type II subunit gamma, CaM kinase II subunit gamma, CaMK-II subunit gamma, CAMK2G, CAMK, CAMK-II, CAMKG|
|Target/Specificity||This CAMK2G (CAMK2 gamma) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-338 amino acids from the C-terminal region of human CAMK2G (CAMK2 gamma).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CAMK2G (CAMK2 gamma) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CAMK, CAMK-II, CAMKG|
|Function||Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in sarcoplsamic reticulum Ca(2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow- twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In neurons, may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning.|
|Cellular Location||Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side|
|Tissue Location||Expressed in skeletal muscle.|
Author : Jiang X1,2,Wu Z1,2,Lu X1,2,Zhang X1,Yu Q1,2,Gan Y1,2,Wu B1,2,Xu Y1,2,Zheng W3,Zhang L1,2,Xu F2,Ma A4,Gan X4,Huang S5,Yu X2,Huang W6,7,Xu R1,2.
Oncotarget. 2017 Aug 24;8(43):75050-75064. doi: 10.18632/oncotarget.20504. eCollection 2017 Sep 26.
Provided below are standard protocols that you may find useful for product applications.
CaM-kinase II (CAMK2) is a prominent Ser/Thr protein kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Likely autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state. CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.
Breen, M.A., et al., Biochem. Biophys. Res. Commun. 236(2):473-478 (1997). Tombes, R.M., et al., Biochim. Biophys. Acta 1355(3):281-292 (1997).