|Application ||WB, IHC-P, E|
|Calculated MW||42914 Da|
|Other Names||Calcium/calmodulin-dependent protein kinase type 1D, CaM kinase I delta, CaM kinase ID, CaM-KI delta, CaMKI delta, CaMKID, CaMKI-like protein kinase, CKLiK, CAMK1D, CAMKID|
|Target/Specificity||This CAMK1D (CAMK1 delta) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-333 amino acids from the C-terminal region of human CAMK1D (CAMK1 delta).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CAMK1D (CAMK1 delta)Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, activates CREB-dependent gene transcription, regulates calcium-mediated granulocyte function and respiratory burst and promotes basal dendritic growth of hippocampal neurons. In neutrophil cells, required for cytokine- induced proliferative responses and activation of the respiratory burst. Activates the transcription factor CREB1 in hippocampal neuron nuclei. May play a role in apoptosis of erythroleukemia cells. In vitro, phosphorylates transcription factor CREM isoform Beta.|
|Cellular Location||Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Nuclear localization increases upon activation by KCl treatment in hippocampal neurons|
|Tissue Location||Widely expressed. Highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes.|
CAMK1-like protein kinase belongs to a proposed calcium-triggered signaling cascade. This protein is expressed in polymorphonuclear leukocytes (PMNs) and may be part of the chemokine signal transduction pathway that regulates granulocyte function. CAMK1-like protein kinase may play a role in apoptosis of erythroleukemia cells. It activates MAP kinase MAPK3, and in vitro, phosphorylates transcription factor CREM isoform Beta and probably CREB1.
Verploegen, S., et al., Blood 96(9):3215-3223 (2000).
Ishikawa, Y., et al., FEBS Lett. 550 (1-3), 57-63 (2003).