- 文献引用 : 1
|Application ||WB, IHC-P, E|
|Calculated MW||89003 Da|
|Other Names||Serine/threonine-protein kinase MARK1, MAP/microtubule affinity-regulating kinase 1, PAR1 homolog c, Par-1c, Par1c, MARK1 (HGNC:6896)|
|Target/Specificity||This MARK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-40 amino acids from the N-terminal region of human MARK1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MARK1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2, MAP4 and MAPT/TAU. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).|
|Cellular Location||Cell membrane; Peripheral membrane protein Cytoplasm, cytoskeleton. Note=Appears to localize to an intracellular network.|
|Tissue Location||Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney.|
Author : Hezel AF, Gurumurthy S, Granot Z, Swisa A, Chu GC, Bailey G, Dor Y, Bardeesy N, Depinho RA.
Mol Cell Biol. 2008 Apr;28(7):2414-25. doi: 10.1128/MCB.01621-07. Epub 2008 Jan 28.
Provided below are standard protocols that you may find useful for product applications.
MARK is a family of kinases that is known for its involvement in establishing cell polarity and in phosphorylating tau protein during Alzheimer neurodegeneration. Expression of MARK causes the phosphorylation of MAPs at their KXGS motifs, thereby detaching MAPs from the microtubules and thus facilitating the transport of particles. This occurs without impairing the intrinsic activity of motors because the velocity during active movement remains unchanged. In primary retinal ganglion cells, transfection with tau leads to the inhibition of axonal transport of mitochondria, APP vesicles, and other cell components which leads to starvation of axons and vulnerability against stress. This transport inhibition can be rescued by phosphorylating tau with MARK
Drewes, G., et al., Cell 89(2):297-308 (1997).