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>   首页   >   产品   >   一抗   >   信号转导   >   Glycerol kinase (GPK2) Antibody (C-term)   

Glycerol kinase (GPK2) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Glycerol kinase (GPK2) Antibody (C-term) AP7131b
    The anti-GKP2 Pab (Cat. #AP7131b) is used in Western blot to detect GKP2 in Jurkat tissue lysate
  • 14 - Glycerol kinase (GPK2) Antibody (C-term) AP7131b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q14410
Other Accession P32189, Q0IID9, Q4R4D5, Q14409
Reactivity Human
Predicted Monkey, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 60594 Da
Additional info
Gene ID 2712
Other Names Glycerol kinase 2, GK 2, Glycerokinase 2, ATP:glycerol 3-phosphotransferase 2, Glycerol kinase, testis specific 2, GK2, GKP2, GKTA
Target/Specificity This Glycerol kinase (GPK2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human Glycerol kinase (GPK2).
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGlycerol kinase (GPK2) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GK2
Synonyms GKP2, GKTA
Function Key enzyme in the regulation of glycerol uptake and metabolism.
Cellular Location Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Note=In sperm the majority of the enzyme is bound to mitochondria.
Research Areas

BACKGROUND

The human glycerol kinase gene family consists of at least 3 expressed loci. The GK1 locus on Xp21.3 is the site of mutations (deletions) causing glycerol kinase deficiency. It comprises 19 exons and is probably ancestral to several other genes which, because they are intronless, are suspected of having arisen by reverse transcriptase mediated events. These include 2 genes on chromosome 4. They are expressed as a single mRNA species in testis where expression is at a high level.

REFERENCES

Sargent, C.A., et al., Hum. Mol. Genet. 3(8):1317-1324 (1994).

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