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BRDT Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - BRDT Antibody (N-term) AP7115a
    All lanes : Anti-BRDT N-term at 1:2000 dilution Lane 1: Human testis lysate Lane 2: PC-3 whole cell lysate Lane 3: MDA-MB-453 whole cell lysate Lane 4: NCI-H1299 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 108 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q58F21
Other Accession Q4R8Y1, O14789
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 107954 Da
Additional info
Gene ID 676
Other Names Bromodomain testis-specific protein, Cancer/testis antigen 9, CT9, RING3-like protein, BRDT
Target/Specificity This BRDT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human BRDT.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBRDT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BRDT
Function Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.
Cellular Location Nucleus Note=Detected on chromatin
Tissue Location Testis-specific. A 3-fold higher expression is seen in adult testis than in embryo testis. Expression seems to be correlated with histone H4 hyperacetylation during the haploid phase of spermatogenesis (spermiogenesis). No expression, or very low expression is seen in patients' testes with abnormal spermatogenesis. Expressed in cancers such as non-small cell lung cancer and squamous cell carcinomas of the head and neck as well as of esophagus, but not in melanoma or in cancers of the colon, breast, kidney and bladder.
Research Areas
Whole-exome sequencing identified a homozygous BRDT mutation in a patient with acephalic spermatozoa.
Author : Li L1,Sha Y2,Wang X3,Li P2,Wang J4,Kee K1,Wang B3.
Oncotarget. 2017 Mar 21;8(12):19914-19922. doi: 10.18632/oncotarget.15251.
28199965

BACKGROUND

BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Two transcript variants encoding the same protein have been found for this gene. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.

REFERENCES

Pivot-Pajot, C., et al., Mol. Cell. Biol. 23(15):5354-5365 (2003).
Dhalluin, C., et al., Nature 399(6735):491-496 (1999).
Jones, M.H., et al., Genomics 45(3):529-534 (1997).

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