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RS18 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - RS18 Antibody (Center) AP6887c
    Overlay histogram showing U-2 OS cells stained with AP6887C(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6887C, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - RS18 Antibody (Center) AP6887c
    All lanes : Anti-RS18 Antibody (Center) at 1:1000 dilution Lane 1: U-251 MG whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: THP-1 whole cell lysate Lane 4: Daudi whole cell lysate Lane 5: Mouse brain lysate Lane 6: Mouse spleen lysate Lane 7: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 18 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - RS18 Antibody (Center) AP6887c
    Formalin-fixed and paraffin-embedded human colon carcinoma reacted with RS18 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, IHC-P, E
Primary Accession P62269
Other Accession P62271, P62272, P62270, Q3T0R1
Reactivity Human, Mouse, Rat
Predicted Rat, Pig, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 6222
Other Names 40S ribosomal protein S18, Ke-3, Ke3, RPS18, D6S218E
Target/Specificity This RS18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the Central region of human RS18.
Dilution FC~~1:25
WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRS18 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPS18
Synonyms D6S218E
Function Located at the top of the head of the 40S subunit, it contacts several helices of the 18S rRNA.
Cellular Location Cytoplasm.
Research Areas

BACKGROUND

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S13P family of ribosomal proteins. It is located in the cytoplasm.

REFERENCES

Yu,Y., Ji,H., et.al., Protein Sci. 14 (6), 1438-1446 (2005)

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