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>   首页   >   产品   >   一抗   >   心血管   >   Calponin-1 Antibody (N-term)   

Calponin-1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Calponin-1 Antibody (N-term) AP6795A
    Western blot analysis of lysates from Hela, MDA-MB-453 cell line (from left to right), using Calponin-1 Antibody (N-term)(Cat. #AP6795a). AP6795a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • 1 - Calponin-1 Antibody (N-term) AP6795A
    Western blot analysis of Calponin-1 Antibody (N-term) (Cat. #AP6795a) in NCI-H460 cell line lysates (35ug/lane). CNN1 (arrow) was detected using the purified Pab.
  • 14 - Calponin-1 Antibody (N-term) AP6795A
    Formalin-fixed and paraffin-embedded human lung carcinoma with Calponin-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - Calponin-1 Antibody (N-term) AP6795A
    Flow cytometric analysis of NCI-H292 cells using Calponin-1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, IHC-P, E
Primary Accession P51911
Other Accession Q08290, Q08091, Q2HJ38
Reactivity Human
Predicted Mouse, Rat, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 1264
Other Names Calponin-1, Basic calponin, Calponin H1, smooth muscle, CNN1
Target/Specificity This Calponin-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-39 amino acids from the N-terminal region of human Calponin-1.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCalponin-1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CNN1
Function Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).
Tissue Location Smooth muscle, and tissues containing significant amounts of smooth muscle
Research Areas
Actin cytoskeleton mediates BMP2-Smad signaling via calponin 1 in preosteoblast under simulated microgravity.
Author : Xu H1,Wu F1,Zhang H1,Yang C1,Li K1,Wang H1,Yang H1,Liu Y1,Ding B1,Tan Y1,Yuan M1,Li Y2,Dai Z3.
Biochimie. 2017 Apr 27. pii: S0300-9084(17)30110-4. doi: 10.1016/j.biochi.2017.04.015. [Epub ahead of print]
28457943

BACKGROUND

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).

REFERENCES

Marsh,W.L., et.al., Appl. Immunohistochem. Mol. Morphol. 17 (3), 216-219 (2009)

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