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AMT Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - AMT Antibody (N-term) AP6739a
    Western blot analysis of AMT Antibody (N-term) (Cat. #AP6739a) in HepG2 cell line lysates (35ug/lane). AMT (arrow) was detected using the purified Pab.
  • 14 - AMT Antibody (N-term) AP6739a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with AMT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - AMT Antibody (N-term) AP6739a
    AMT Antibody (N-term) (Cat.#AP6739a) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession P48728
Other Accession Q8CFA2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 43946 Da
Additional info
Gene ID 275
Other Names Aminomethyltransferase, mitochondrial, Glycine cleavage system T protein, GCVT, AMT, GCST
Target/Specificity This AMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-45 amino acids from the N-terminal region of human AMT.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAMT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AMT (HGNC:473)
Function The glycine cleavage system catalyzes the degradation of glycine.
Cellular Location Mitochondrion.
Research Areas

BACKGROUND

The enzyme system for cleavage of glycine (glycine cleavage system; EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase; MIM 238300), H protein (a lipoic acid-containing protein; MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase; MIM 238331). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes.

REFERENCES

Nanao,K., Genomics 19 (1), 27-30 (1994)

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