|Application ||WB, IHC-P, FC, E|
|Calculated MW||43946 Da|
|Other Names||Aminomethyltransferase, mitochondrial, Glycine cleavage system T protein, GCVT, AMT, GCST|
|Target/Specificity||This AMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-45 amino acids from the N-terminal region of human AMT.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||AMT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The glycine cleavage system catalyzes the degradation of glycine.|
Provided below are standard protocols that you may find useful for product applications.
The enzyme system for cleavage of glycine (glycine cleavage system; EC 18.104.22.168), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase; MIM 238300), H protein (a lipoic acid-containing protein; MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase; MIM 238331). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes.
Nanao,K., Genomics 19 (1), 27-30 (1994)