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Annexin V Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 59 - Annexin V Antibody (N-term) AP6580a
    Immunohistochemical analysis of paraffin-embedded Human placenta tissue using AP6580A performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 59 - Annexin V Antibody (N-term) AP6580a
    Immunohistochemical analysis of paraffin-embedded Human hepatocarcinoma tissue using AP6580A performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - Annexin V Antibody (N-term) AP6580a
    All lanes : Anti-Annexin V Antibody (N-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: 293 whole cell lysate Lane 3: U-2OS whole cell lysate Lane 4: A2058 whole cell lysate Lane 5: T47D whole cell lysate Lane 6: Mouse lung lysate Lane 7: Mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 4 - Annexin V Antibody (N-term) AP6580a
    Annexin V Antibody (N-term) (Cat. #AP6580a) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, FC, WB, E
Primary Accession P08758
Other Accession Q4R4H7, P81287
Reactivity Human, Mouse
Predicted Bovine, Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35937 Da
Additional info
Gene ID 308
Other Names Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, CBP-I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, PP4, Placental anticoagulant protein I, PAP-I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, VAC-alpha, ANXA5, ANX5, ENX2, PP4
Target/Specificity This Annexin V antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Annexin V.
Dilution IHC-P-Leica~~1:500
WB~~1:2000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAnnexin V Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ANXA5
Synonyms ANX5, ENX2, PP4
Function This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Research Areas

BACKGROUND

Annexin 5 belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

REFERENCES

Tiscia,G., Thromb. Haemost. 102 (2), 309-313 (2009)
Kim,T.H., Bone 45 (1), 125-131 (2009)

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