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EEF1D Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - EEF1D Antibody (N-term) AP6523a
    Western blot analysis of EEF1D antibody (N-term) (Cat.# AP6523a) in Ramos cell line lysates (35ug/lane). EEF1D (arrow) was detected using the purified Pab.
  • 14 - EEF1D Antibody (N-term) AP6523a
    Formalin-fixed and paraffin-embedded human lymph reacted with EEF1D Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - EEF1D Antibody (N-term) AP6523a
    EEF1D Antibody (N-term) (Cat. #AP6523a) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession P29692
Other Accession Q68FR9, P57776, Q4R3D4
Reactivity Human
Predicted Monkey, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 31122 Da
Additional info
Gene ID 1936
Other Names Elongation factor 1-delta, EF-1-delta, Antigen NY-CO-4, EEF1D, EF1D
Target/Specificity This EEF1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EEF1D.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEEF1D Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EEF1D
Synonyms EF1D
Function Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1- alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.
Cellular Location Isoform 2: Nucleus
Tissue Location Isoform 2 is specifically expressed in brain, cerebellum and testis
Research Areas

BACKGROUND

EEF1D is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs.

REFERENCES

Yang,S., BMC Cancer 7, 211 (2007)
Mulner-Lorillon,O., J. Biol. Chem. 269 (31), 20201-20207 (1994)

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